Information on EC 2.3.1.103 - sinapoylglucose-sinapoylglucose O-sinapoyltransferase

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The expected taxonomic range for this enzyme is: Raphanus sativus

EC NUMBER
COMMENTARY hide
2.3.1.103
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RECOMMENDED NAME
GeneOntology No.
sinapoylglucose-sinapoylglucose O-sinapoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 1-O-sinapoyl beta-D-glucoside = D-glucose + 1,2-bis-O-sinapoyl beta-D-glucoside
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
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sinapoyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sinapate ester biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
1-O-(4-hydroxy-3,5-dimethoxycinnamoyl)-beta-D-glucoside:1-O-(4-hydroxy-3,5-dimethoxycinnamoyl-beta-D-glucoside 1-O-sinapoyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
103537-11-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
L. var. sativus cv. Saxa
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(p-coumaroyl)-beta-D-glucose + 1-(p-coumaroyl)-beta-D-glucose
1,2-di-O-p-coumaroyl-beta-D-glucopyranose + D-glucose
show the reaction diagram
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-
-
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ir
1-feruloyl-beta-D-glucose + 1-feruloyl-beta-D-glucose
1,2-di-O-feruloyl-beta-glucopyranose + glucose
show the reaction diagram
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-
-
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ir
1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose
1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
show the reaction diagram
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-
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ir
additional information
?
-
-
strict specificity of acyl transfer to the C2 hydroxyl group of the acceptor glucose molecule
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose
1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
show the reaction diagram
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-
-
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ir
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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1 mM, total inhibition
Ca2+
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divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 45% remaining activity
Co2+
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divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, total inhibition
dithiothreitol
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1 mM, total inhibition
Mg2+
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divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 65% remaining activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no requirement for divalent cations or thiols
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.58
1-(p-coumaroyl)-glucose
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-
0.4
1-feruloyl-beta-D-glucose
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0.42
1-sinapoyl-beta-D-glucose
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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HEPES-buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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50% of maximal activity at pH 7.0 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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dark grown seedlings show higher activity than light grown seedlings
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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gel filtration, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 55000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, presence of 3 mg/ml bovine serum albumin, at least 1 year
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE