Information on EC 2.3.1.130 - galactarate O-hydroxycinnamoyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY hide
2.3.1.130
-
RECOMMENDED NAME
GeneOntology No.
galactarate O-hydroxycinnamoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
feruloyl-CoA + galactarate = CoA + O-feruloylgalactarate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
feruloyl-CoA:galactarate O-(hydroxycinnamoyl)transferase
Sinapoyl-CoA and 4-coumaroyl-CoA can also act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
112956-50-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye, var. Kustro
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeoyl-CoA + galactarate
CoA + O-caffeoylgalactarate
show the reaction diagram
-
poor substrate
-
-
?
feruloyl-CoA + galactarate
CoA + O-feruloylgalactarate
show the reaction diagram
-
best substrate, no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
-
?
p-coumaroyl-CoA + galactarate
CoA + O-p-coumaroylgalactarate
show the reaction diagram
-
condensation at 69% the rate of the reaction with feruloyl-CoA
-
-
?
sinapoyl-CoA + galactarate
CoA + O-sinapoylgalactarate
show the reaction diagram
-
condensation at 78% the rate of the reaction with feruloyl-CoA
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
Feruloyl-CoA
-
-
33
galactarate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0033
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
25 mM potassium phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 7.6
-
about 80% of maximal activity at pH 7.1 and 7.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
primary
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
-
denaturation after 5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE