Information on EC 2.3.1.131 - glucarate O-hydroxycinnamoyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY hide
2.3.1.131
-
RECOMMENDED NAME
GeneOntology No.
glucarate O-hydroxycinnamoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sinapoyl-CoA + glucarate = CoA + O-sinapoylglucarate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
hydroxycinnamate sugar acid ester biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
sinapoyl-CoA:glucarate O-(hydroxycinnamoyl)transferase
4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
112956-51-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye, var. Kustro
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeoyl-CoA + glucarate
CoA + caffeoylglucarate
show the reaction diagram
-
condensation at 8% the rate of the reaction with sinapoyl-CoA
-
?
feruloyl-CoA + glucarate
CoA + feruloylglucarate
show the reaction diagram
-
condensation at 54% the rate of the reaction with sinapoyl-CoA
-
?
p-coumaroyl-CoA + glucarate
CoA + p-coumaroylglucarate
show the reaction diagram
-
condensation at 85% the rate of the reaction with sinapoyl-CoA
-
?
sinapoyl-CoA + glucarate
CoA + O-sinapoylglucarate
show the reaction diagram
-
best substrate
-
?
additional information
?
-
-
no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
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-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8
glucarate
-
-
0.018
Sinapoyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
feruloyl-CoA as donor
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
100 mM potassium phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 8
-
about 80% of maximal activity at pH 6.2 and 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
primary
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
-
denaturation after 5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE