Information on EC 2.3.1.132 - glucarolactone O-hydroxycinnamoyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY hide
2.3.1.132
-
RECOMMENDED NAME
GeneOntology No.
glucarolactone O-hydroxycinnamoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sinapoyl-CoA + glucarolactone = CoA + O-sinapoylglucarolactone
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
hydroxycinnamate sugar acid ester biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
sinapoyl-CoA:glucarolactone O-(hydroxycinnamoyl)transferase
4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
112956-52-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye, var. Kustro
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeoyl-CoA + glucarolactone
CoA + O-caffeoylglucarolactone
show the reaction diagram
-
poor substrate
-
?
feruloyl-CoA + glucarolactone
CoA + O-feruloylglucarolactone
show the reaction diagram
-
condensation at 37% the rate of the reaction with sinapoyl-CoA
-
?
p-coumaroyl-CoA + glucarolactone
CoA + O-p-coumaroylglucarolactone
show the reaction diagram
-
condensation at 54% the rate of the reaction with sinapoyl-CoA
-
?
sinapoyl-CoA + glucarolactone
CoA + O-sinapoylglucarolactone
show the reaction diagram
-
best substrate
-
?
additional information
?
-
-
no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sinapoyl-CoA + glucarolactone
CoA + O-sinapoylglucarolactone
show the reaction diagram
-
best substrate
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
-
activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
glucarolactone
-
-
0.029
Sinapoyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
50 mM potassium phosphate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 8.2
-
about 80% of maximal activity at pH 7.4 and pH 8.2
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
-
denaturation after 5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE