Information on EC 2.3.1.143 - beta-glucogallin-tetrakisgalloylglucose O-galloyltransferase

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The expected taxonomic range for this enzyme is: Quercus rubra

EC NUMBER
COMMENTARY hide
2.3.1.143
-
RECOMMENDED NAME
GeneOntology No.
beta-glucogallin-tetrakisgalloylglucose O-galloyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-O-galloyl-beta-D-glucose + 1,2,3,6-tetrakis-O-galloyl-beta-D-glucose = D-glucose + 1,2,3,4,6-pentakis-O-galloyl-beta-D-glucose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pentagalloylglucose biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
1-O-galloyl-beta-D-glucose:1,2,3,6-tetrakis-O-galloyl-beta-D-glucose 4-O-galloyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
122653-70-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
oak
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-O-galloyl-beta-D-glucose + 1,2,3,6-tetra-O-galloyl-beta-D-glucose
D-glucose + 1,2,3,4,6-penta-O-galloyl-beta-D-glucose
show the reaction diagram
additional information
?
-
-
the enzyme catalyzes the last common step in the biosynthesis of hydrolyzable tannins
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme catalyzes the last common step in the biosynthesis of hydrolyzable tannins
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
1,2,3,6-tetra-O-galloyl-beta-D-glucose
-
-
2.3
1-O-Galloyl-beta-D-glucose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
-
50% of maximal activity at pH 4.0 and pH 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
some activity at 0°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
260000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
-
4 * 65000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.5
-
-
486327
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, within 1-2 weeks no significant loss of activity
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive, buffers have to be supplemented with 5 mM 2-mercaptoethanol
-
486327
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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