Information on EC 2.3.1.145 - piperidine N-piperoyltransferase

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The expected taxonomic range for this enzyme is: Piper nigrum

EC NUMBER
COMMENTARY hide
2.3.1.145
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RECOMMENDED NAME
GeneOntology No.
piperidine N-piperoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(E,E)-piperoyl-CoA + piperidine = CoA + N-[(E,E)-piperoyl]-piperidine
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
piperine biosynthesis
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Tropane, piperidine and pyridine alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(E,E)-piperoyl-CoA:piperidine N-piperoyltransferase
Pyrrolidine and 3-pyrroline can also act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
126806-22-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E,E)-piperoyl-CoA + piperidine
CoA + N-[(E,E)-piperoyl]-piperidine
show the reaction diagram
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-
-
-
?
piperoyl-CoA + 3-pyrroline
CoA + piperoyl-(3-pyrroline)
show the reaction diagram
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25% of activity with piperidine
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?
piperoyl-CoA + pyrrolidine
CoA + piperoylpyrrolidine
show the reaction diagram
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76% of activity with piperidine
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(E,E)-piperoyl-CoA + piperidine
CoA + N-[(E,E)-piperoyl]-piperidine
show the reaction diagram
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-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
piperidine
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3.8
Piperoyl-CoA
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 42
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heat-denaturation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
10 mM 2-mercaptoethanol stabilizes
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preparations are to be carried out at 0-4°C
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
cell homogenate has to be supplemented with 50 mM ascorbic acid as antioxidant
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486332
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, enzyme stored as ammonium sulfate paste loses 20-25% of activity within one week, completely inactive after two weeks
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diluted enzyme stored after anion-exchange chromatography is unstable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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