Information on EC 2.3.1.149 - Platelet-activating factor acetyltransferase

Word Map on EC 2.3.1.149
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.149
-
RECOMMENDED NAME
GeneOntology No.
Platelet-activating factor acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid = 1-alkyl-2-lyso-sn-glycero-3-phosphocholine + 1-organyl-2-acetyl-sn-glycero-3-phospholipid
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
1-alkyl-2-acyl-sn-glycero-3-phosphocholine:1-organyl-2-lyso-sn-glycero-3-phospholipid acetyltransferase
Catalyses the transfer of the acetyl group from 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) to the sn-2 position of lyso-glycerophospholipids containing ethanolamine, choline, serine, inositol or phosphate groups at the sn-3 position as well as to sphingosine and long-chain fatty alcohols. The organyl group can be alkyl, acyl or alk-1-enyl (sometimes also collectively referred to as 'radyl').
CAS REGISTRY NUMBER
COMMENTARY hide
9012-30-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
lyso- platelet-activating factor accumulation and an increase in platelet-activating factor bioactivity is observed in the aorta of some patients. In atherogenic aortas, an imbalance between PAF-AH and transacetylase activity, as well as lyso-platelet-activating factor accumulation, may lead to unregulated platelet-activating factor formation and to progression of atherosclerosis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-acyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid
1-organyl-sn-glycero-3-phosphocholine + 1-acyl-2-lyso-sn-glycero-3-phospholipid
show the reaction diagram
-
1-acyl-sn-glycero-3-phosphocholine is termed lyso-PC
-
-
?
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + acyllyso-sn-glycero-3-phosphate
?
show the reaction diagram
-
-
-
-
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + acyllyso-sn-glycero-3-phosphoinositol
?
show the reaction diagram
-
-
-
-
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + acyllyso-sn-glycero-3-phosphoserine
?
show the reaction diagram
-
-
-
-
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + alkyllyso-sn-glycero-3-phosphate
?
show the reaction diagram
-
-
-
-
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + cis-9-octadecen-1-ol
?
show the reaction diagram
-
-
-
-
-
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + lyso-phosphatidylcholine
?
show the reaction diagram
-
-
-
-
?
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + lysoplasmalogen
1-Alk-1-enyl-2-acetyl-sn-glycero-3-phosphoethanolamine + ?
show the reaction diagram
-
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine is platelet-activating factor
-
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + radyllyso-sn-glycero-3-phosphocholine
?
show the reaction diagram
-
-
-
-
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphocholine + radyllyso-sn-glycero-3-phosphoethanolamine
?
show the reaction diagram
-
-
-
-
-
1-hexadecanoyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid
1-organyl-sn-glycero-3-phosphocholine + 1-O-hexadecanoyl-2-lyso-sn-glycero-3-phospholipid
show the reaction diagram
-
1-hexadecanoyl-sn-glycero-3-phosphocholine is termed C16:0 lyso-PC
-
-
?
1-O-alkyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid
1-organyl-sn-glycero-3-phosphocholine + 1-O-alkyl-2-lyso-sn-glycero-3-phospholipid
show the reaction diagram
-
1-O-alkyl-sn-glycero-3-phosphocholine is termed lyso-PAF, artificial substrate
-
-
?
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + 1-organyl-2-lyso-sn-glycero-3-phospholipid
1-organyl-sn-glycero-3-phosphocholine + 1-O-hexadecyl-2-lyso-sn-glycero-3-phospholipid
show the reaction diagram
-
1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine is termed C16:0 lyso-PAF
-
-
?
Hexadecyl-acetyl-sn-glycero-3-phosphocholine + alk-1-enyllyso-sn-glycero-phosphoethanolamine
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
enzyme activities are decreased upon oxidation of LDL with Cu2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-(2-aminoethyl)benzene-sulfonyl fluoride
-
i.e. Pefabloc, complete inhibition of both enzyme activities at 0.05 mM
diisopropylfluorophosphate
-
complete inhibition of both enzyme activities at 20 mM
ether/ester-linked lysophospholipids
-
competitive
-
Mepacrine
-
-
p-bromophenacyl bromide
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1064
alk-1-enyllyso-sn-glycero-phosphoethanolamine
-
-
0.012
Hexadecyl-acetyl-sn-glycero-3-phosphocholine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000008
-
transacetylase activity of mammary artery
0.0000033
-
transacetylase activity in aorta
additional information
-
activities of different LDL subfractions
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 24
-
active between 15°C and 24°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immunohistochemistry shows PAF-AH expression in the intima and in the media of the aorta
Manually annotated by BRENDA team
-
detected by immunohistochemistry in the media of mammary arteries
Manually annotated by BRENDA team
-
purified recombinant enzyme
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
isolated from human plasma, different subfractions
-
Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
60 min, loss of 10% transacetylase activity, acetylhydrolase activity is completely stable
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme activities are decreased upon oxidation of LDL with Cu2+
-
649713