Information on EC 2.3.1.222 - phosphate propanoyltransferase

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The expected taxonomic range for this enzyme is: Salmonella enterica

EC NUMBER
COMMENTARY hide
2.3.1.222
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RECOMMENDED NAME
GeneOntology No.
phosphate propanoyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
propanoyl-CoA + phosphate = CoA + propanoyl phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-1,2-propanediol degradation
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L-threonine degradation I
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threonine metabolism
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Propanoate metabolism
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SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:phosphate propanoyltransferase
Part of the degradation pathway for propane-1,2-diol .
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
serovar typhimurium LT2, diverse strains, overview, gene pduL
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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PduL homologues are distributed among at least 49 bacterial species but are absent from the Archaea and Eukarya. Sequence analyses show that PduL is unrelated in amino acid sequence to known phosphotransacylase, PTAC, enzymes
malfunction
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pduL mutants are unable to ferment 1,2-propanediol and are also impaired for aerobic growth on this compound, overview. Ectopic expression of pduL corrects the growth defects of a pta mutant, the pta gene encodes another PTAC enzyme
metabolism
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PduL is required in the 1,2-propandiol degradation pathway, overview
physiological function
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PduL is a phosphotransacylase, PTAC, required for 1,2-propanediol degradation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + phosphate
CoA + acetyl phosphate
show the reaction diagram
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r
CoA + acetyl phosphate
acetyl-CoA + phosphate
show the reaction diagram
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PduL-His8 is 3.8fold more active with propionyl phosphate than with acetyl phosphate
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r
CoA + propanoyl phosphate
propanoyl-CoA + phosphate
show the reaction diagram
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PduL-His8 is 3.8fold more active with propionyl phosphate than with acetyl phosphate
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r
propanoyl-CoA + phosphate
CoA + propanoyl phosphate
show the reaction diagram
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r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
propanoyl-CoA + phosphate
CoA + propanoyl phosphate
show the reaction diagram
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r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.97
acetyl phosphate
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reverse reaction, pH and temperature not specified in the publication
0.61
propanoyl phosphate
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reverse reaction, pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.4
acetyl phosphate
Salmonella enterica
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reverse reaction, pH and temperature not specified in the publication
20.7
propanoyl phosphate
Salmonella enterica
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reverse reaction, pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.6
acetyl phosphate
Salmonella enterica
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reverse reaction, pH and temperature not specified in the publication
358
33.9
propanoyl phosphate
Salmonella enterica
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reverse reaction, pH and temperature not specified in the publication
10758
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.9
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wild-type strain, pH and temperature not specified in the publication
50
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recombinant His8-tagged PduL in soluble fraction of the expressing Escherichia coli strain BE554, pH and temperature not specified in the publication
PDB
SCOP
CATH
ORGANISM
UNIPROT
Rhodopseudomonas palustris (strain BisB18)
Rhodopseudomonas palustris (strain BisB18)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 27000, recombinant His8-tagged PduL, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His8-tagged PduL from Escherichia coli strain BE554 soluble fraction by nickel affinity chromatographhy
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene pduL, DNA and amino acid sequence determination and analysis, sequence comparison, expression as His8-tagged protein in Escherichia coli strain BE554 mostly in inclusion bodies
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