Information on EC 2.3.1.224 - acetyl-CoA-benzylalcohol acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.224
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RECOMMENDED NAME
GeneOntology No.
acetyl-CoA-benzylalcohol acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + benzyl alcohol = CoA + benzyl acetate
show the reaction diagram
acetyl-CoA + cinnamyl alcohol = CoA + cinnamyl acetate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
epoxypseudoisoeugenol-2-methylbutanoate biosynthesis
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eugenol and isoeugenol biosynthesis
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phenylethyl acetate biosynthesis
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volatile benzenoid biosynthesis I (ester formation)
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:benzylalcohol O-acetyltransferase
The enzyme is found in flowers like Clarkia breweri, where it is important for floral scent production. Unlike EC 2.3.1.84, alcohol O-acetyltransferase, this enzyme is active with alcohols that contain a benzyl ring.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is important for floral scent production
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 2-(naphthalen-1-yl)ethanol
CoA + 2-(naphthalen-1-yl)ethyl acetate
show the reaction diagram
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139% activity compared to benzyl alcohol with isoform BEAT-1, 93% activity compared to benzyl alcohol with isoform BEAT-2, 217% activity compared to benzyl alcohol with isoform BEAT-3
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-
?
acetyl-CoA + 2-(naphthalen2-yl)ethanol
CoA + 2-(naphthalen2-yl)ethyl acetate
show the reaction diagram
at 9% of the activity compared to benzyl alcohol, plant purified enzyme, at 28% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme)
-
-
?
acetyl-CoA + 2-phenylethanol
CoA + 2-phenylethyl acetate
show the reaction diagram
acetyl-CoA + 2-phenylethanol
CoA + 2-phenylethylacetate
show the reaction diagram
at 33% of the activity compared to benzyl alcohol, plant purified enzyme, at 57% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme)
-
-
?
acetyl-CoA + 3-cis-hexene-1-ol
CoA + (3Z)-hex-3-en-1-yl acetate
show the reaction diagram
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
show the reaction diagram
acetyl-CoA + cinnamyl alcohol
CoA + cinnamyl acetate
show the reaction diagram
acetyl-CoA + heptanol
CoA + heptyl acetate
show the reaction diagram
acetyl-CoA + naphthalene ethanol
?
show the reaction diagram
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57% activity compared to benzyl alcohol with isoform BEAT-1, 50% activity compared to benzyl alcohol with isoform BEAT-2
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-
?
malonyl-CoA + cyanidin 3-O-(6''-O-4-coumarylglucoside)-5-O-(6'''-O-malonylglucoside)
CoA + ?
show the reaction diagram
-
-
-
?
malonyl-CoA + monodemalonylsalvianin
CoA + salvianin
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + benzyl alcohol
CoA + benzyl acetate
show the reaction diagram
acetyl-CoA + cinnamyl alcohol
CoA + cinnamyl acetate
show the reaction diagram
O64988
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-
-
?
malonyl-CoA + cyanidin 3-O-(6''-O-4-coumarylglucoside)-5-O-(6'''-O-malonylglucoside)
CoA + ?
show the reaction diagram
Q6TXD2
-
-
-
?
malonyl-CoA + monodemalonylsalvianin
CoA + salvianin
show the reaction diagram
Q6TXD2
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-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
32% residual activity at 0.1 mM
bisdemalonylsalvianin
31% residual activity at 0.05 mM
caffeic acid
38% residual activity at 1 mM
Cd2+
complete inhibition at 0.1 mM
CoA-SH
complete inhibition at 0.1 mM
Cu2+
complete inhibition at 0.1 mM
diethyldicarbonate
69% residual activity at 0.1 mM
Fe2+
complete inhibition at 0.1 mM
Hg+
complete inhibition at 0.1 mM
N-ethylmaleimide
complete inhibition at 5 mM
Zn2+
complete inhibition at 0.1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
acetyl-CoA
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in 250 mM Tris-HCl pH 7.5, 25 mM MgCl2, 25 mM KC1, 10 mM 2-mercaptoethanol, at 30C
0.12 - 0.34
benzyl alcohol
0.0046 - 0.0069
malonyl-CoA
0.0513 - 0.0563
monodemalonylsalvianin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55 - 0.76
malonyl-CoA
0.76
monodemalonylsalvianin
Salvia splendens
Q6TXD2
recombinant enzyme, at pH 7.0 and 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
79.7 - 165
malonyl-CoA
76
10.7 - 13.5
monodemalonylsalvianin
19762
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000047
crude extract, at pH 7.0 and 30C
0.0024
after 510fold purification, at pH 7.0 and 30C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
no BEAT mRNA is detected in leaves
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
1 * 58000, SDS-PAGE; x * 48200, calculated from sequence
monomer
1 * 46000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, DEAE-Sepharose column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli JM109 cells
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the levels of BEAT mRNA in the petals increases as the bud matures, and peaks at anthesis
three days after anthesis, mRNA levels steeply declines