Information on EC 2.3.1.229 - 4-coumaroyl-homoserine lactone synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Rhodopseudomonas palustris

EC NUMBER
COMMENTARY hide
2.3.1.229
-
RECOMMENDED NAME
GeneOntology No.
4-coumaroyl-homoserine lactone synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-coumaroyl-CoA + S-adenosyl-L-methionine = CoA + S-methyl-5'-thioadenosine + N-(4-coumaroyl)-L-homoserine lactone
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
4-coumaroyl-CoA:S-adenosyl-L-methionine trans-4-coumaroyltranserase (lactone-forming, methylthioadenosine-releasing)
The enzyme is found in the bacterium Rhodopseudomonas palustris, which produces N-(4-coumaroyl)-L-homoserine lactone as a quorum-sensing signal.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-coumaroyl-CoA + S-adenosyl-L-methionine
CoA + S-methyl-5'-thioadenosine + N-(4-coumaroyl)-L-homoserine lactone
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-coumaroyl-CoA + S-adenosyl-L-methionine
CoA + S-methyl-5'-thioadenosine + N-(4-coumaroyl)-L-homoserine lactone
show the reaction diagram
additional information
?
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the bacterium has a signal receptor with homology to fatty acyl-homoserine lactone receptors that responds to 4-coumaroyl-homoserine lactone to regulate global gene expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
development of an artificial biosynthetic process for phenylacetyl-homoserine lactone analogs, including cinnamoyl-homoserine lactone, 4-coumaroyl-homoserine lactone, caffeoyl-homoserine lactone, and feruloyl-homoserine lactone, using coexpression of the codon-optimized synthase RpaI and 4-coumaroyl-CoA ligase 4CL2nt, EC 6.2.1.12, in Escherichia coli. De novo production of p-coumaroyl-homoserine lactone in Escherichia coli can be achieved by expression of the rpaI gene in addition to 4-coumaroyl-CoA biosynthetic genes. The yields for 4-coumaroyl-homoserine lactone reach 93 and 142 mg/l in the S-adenosyl-L-methionine and L-methionine feeding culture, respectively