Information on EC 2.3.1.235 - tetracenomycin F2 synthase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
2.3.1.235
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RECOMMENDED NAME
GeneOntology No.
tetracenomycin F2 synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
10 malonyl-CoA = tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of type II polyketide backbone
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:acetate malonyltransferase (tetracenomycin F2 forming)
A multi-domain polyketide synthase involved in the synthesis of tetracenomycin in the bacterium Streptomyces glaucescens. It involves a ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). A malonyl-CoA molecule is initially bound to the acyl carrier protein and decarboxylated to form an acetyl starter unit. Additional two-carbon units are added from nine more malonyl-CoA molecules.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the multi-domain polyketide synthase involved in the synthesis of tetracenomycin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10 malonyl-CoA
tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10 malonyl-CoA
tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O
show the reaction diagram
-
the multi-domain polyketide synthase involved in the synthesis of tetracenomycin
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-
?
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the TcmKL KS alphabeta ketosynthase complex, an acyl carrier protein TcmM ACP, TcmN cyclase, and FabD malonyl CoA:ACP acyltransferase proteins constitute the minimal requirements for a functional tetracenomycin polyketide synthase in vitro
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purification of the TcmK/TcmL alphabeta complex
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TcmN polyketide cyclase
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of tcmN in Escherichia coli
overexpression of the tcmKL genes together in Streptomyces lividans to obtain the KS subunits
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C173A
plasmids carrying the C173A mutation produce no detectable amount of tetracenomycin F2
C173S
plasmids carrying the C173S mutation produce no detectable amount of tetracenomycin F2
H350L/S351A
plasmids carrying the H350L/S351A double mutation produce no detectable amount of tetracenomycin F2
S351A
plasmids carrying the S351A mutation produce high amounts of tetracenomycin F2