Information on EC 2.3.1.250 - [Wnt protein] O-palmitoleoyl transferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.250
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RECOMMENDED NAME
GeneOntology No.
[Wnt protein] O-palmitoleoyl transferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine = CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
(9Z)-hexadec-9-enoyl-CoA:[Wnt]-L-serine O-hexadecenoyltransferase
The enzyme, found in animals, modifies a specific serine residue in Wnt proteins, e.g. Ser209 in human Wnt3a and Ser224 in chicken WNT1 [2,3]. The enzyme can accept C13 to C16 fatty acids in vitro, but only (9Z)-hexadecenoate modification is observed in vivo [1]. cf. EC 3.1.1.98, [Wnt protein]-O-palmitoleoyl-L-serine hydrolase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene mom-1; gene mom-1
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene Xporc
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine
CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
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?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5a]-L-serine
CoA + [Wnt5a]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
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?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine
CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
Q9VWV9
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?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5a]-L-serine
CoA + [Wnt5a]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
Q9H237
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?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
Q9VWV9
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?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine
CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
Q9VWV9
the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg
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?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-((3,6-dimethyl-4-oxo-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide
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2-((3-(3-methoxyphenyl)-4-oxo-3,4,4a,6,7,7a-hexahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide
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2-((3-(4-fluorophenyl)-4-oxo-3,4,4a,6,7,7a-hexahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide
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2-((3-cyclopentyl-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(4-(dimethylamino)phenyl)acetamide
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2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(4-(piperidin-1-yl)phenyl)acetamide
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2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(4-phenylthiazol-2-yl)acetamide
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2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(5-(prop-1-en-2-yl)pyridin-2-yl)acetamide
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2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(5-phenylthiazol-2-yl)acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-(5-phenylpyridin-2-yl)acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-(5-phenylpyrimidin-2-yl)acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-(6-phenylpyridin-3-yl)acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-[4-(pyridin-2-yl)phenyl]acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-[4-(pyridin-3-yl)phenyl]acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-[4-(pyridin-4-yl)phenyl]acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-[5-(pyrimidin-5-yl)pyridin-2-yl]acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-[5-(thiophen-2-yl)pyridin-2-yl]acetamide
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2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]-N-[5-(thiophen-3-yl)pyridin-2-yl]acetamide
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5-((3aR,4R,6aS)-6-(4-(2-((2-((6-methylbenzo[d]thiazol-2-yl)amino)-2-oxoethyl)thio)-4-oxo-4a,6,7,7a-tetrahydrothieno[3,2-d]pyrimidin-3(4H)-yl)phenethyl)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid
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6-oxo-N-(2-oxo-2-(thiazol-2-ylamino)ethyl)-1-phenyl-1,6-dihydropyridazine-3-carboxamide
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ethyl 4-(2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamido)benzoate
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LGK974
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N-(2-((6-methoxybenzo[d]thiazol-2-yl)amino)-2-oxoethyl)-3-(4-methoxyphenyl)-4-oxo-3,4-dihydrophthalazine-1-carboxamide
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N-(2-((6-methoxybenzo[d]thiazol-2-yl)amino)-2-oxoethyl)-6-oxo-1-phenyl-1,6-dihydropyridine-3-carboxamide
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N-(3,3'-bipyridin-6-yl)-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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N-(4-(dimethylamino)phenyl)-2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-(4-butylphenyl)-2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-(5-ethoxypyridin-2-yl)-2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-(6-methoxyquinolin-2-yl)-2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-(6-methylbenzo[d]thiazol-2-yl)-2-((4-oxo-3-phenyl-3,4,4a,6,7,7a-hexahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-(benzo[d]thiazol-2-yl)-2-((3-ethyl-6-methyl-4-oxo-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-(biphenyl-2-yl)-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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N-(biphenyl-3-yl)-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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N-([1,1'-biphenyl]-4-yl)-2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide
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N-[5-(furan-2-yl)pyridin-2-yl]-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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N-[5-(furan-2-yl)pyrimidin-2-yl]-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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N-[5-(furan-3-yl)pyridin-2-yl]-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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N-[5-(furan-3-yl)pyrimidin-2-yl]-2-[(4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide
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additional information
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identification, synthesis, and analysis of small molecule enzyme IWP inhibitors, i.e. inhibitors of Wnt production, structure-activity relationship, overview
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
Mporc mRNA is differentially expressed during embryogenesis and in various adult tissues, demonstrating that the alternative splicing is regulated to synthesize the specific types of Mporc, pattern of expression of Mporc at fetal and adult stages of development, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
lipoprotein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene MG61, DNA and amino acid sequence determination and analysis, sequence comparisons
gene mom-1, DNA and amino acid sequence determination and analysis, sequence comparisons
gene Porc, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in HEK-293 cells with or without co-expression of Wg; gene porc, expression of the enzyme tagged with three HA epitopes at amino acid 28. Capped porc, porc3HA28, DELTA34, DELTA66, and DELTA110 RNA are prepared by in vitro transcription and RNA is injected into Drosophila embryos
gene PORCN, DNA and amino acid sequence determination and analysis, genotyping
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gene PORCN, quantitative PCR enzyme expression analysis
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gene PORCN, quantitative RT-PCR enzyme expression analysis. PORCN splice variants differ globally in signaling activity in HT-1080 cells
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gene Xporc, DNA and amino acid sequence determination and analysis, sequence comparisons
overexpression of HA-tagged human PORC to confirm the ability of hPORC RNAi constructs to knockdown hPORC expression
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single gene Mporc, variants by alternative splicing, DNA and amino acid sequence determination and analysis, sequence comparisons, co-expression in COS-1 cells of HA-tagged Mporc and either Myc-tagged Wnt or partial laminin beta chain, recombinant expression in HEK-293 cells with or without co-expression of Drosophila melanogaster Wnt variant Wg
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C187A
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site-directed mutagenesis, increase in Wnt signaling activity of about 1.5fold upon expression of PORCNC187A compared to wild-type PORCN
C375X
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site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant
C381X
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site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant
C385X
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site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 resi­due, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant
H341D
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site-directed mutagenesis, catalytically inactive mutant
H341Q
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site-directed mutagenesis, catalytically inactive mutant
additional information