Information on EC 2.3.1.49 - deacetyl-[citrate-(pro-3S)-lyase] S-acetyltransferase

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The expected taxonomic range for this enzyme is: Enterobacter aerogenes

EC NUMBER
COMMENTARY hide
2.3.1.49
-
RECOMMENDED NAME
GeneOntology No.
deacetyl-[citrate-(pro-3S)-lyase] S-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-acetylphosphopantetheine + deacetyl-[citrate-oxaloacetate-lyase((pro-3S)-CH2COO-->acetate)] = phosphopantetheine + [citrate-oxaloacetate-lyase((pro-3S)-CH2COO-->acetate)]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
citrate lyase activation
-
-
SYSTEMATIC NAME
IUBMB Comments
S-acetylphosphopantetheine:deacetyl-[citrate-oxaloacetate-lyase((pro-3S)-CH2COO-->acetate)] S-acetyltransferase
Both this enzyme and EC 6.2.1.22, [citrate (pro-3S)-lyase] ligase, acetylate and activate EC 4.1.3.6, citrate (pro-3S)-lyase.
CAS REGISTRY NUMBER
COMMENTARY hide
42616-18-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-acetylphosphopantetheine + deacetyl-[citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)]
phosphopantetheine + [citrate-oxaloacetate-lyase((pro-3S)-CH2COO---acetate)]
show the reaction diagram
-
-
i.e. active form of EC 4.1.3.6
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
-
preincubation of the acetyl transferase with 0.4 mM N-ethylmaleimide results in large decrease of active citrate lyase formed
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
25% of maximal activity at pH 6, 95% of maximal activity at pH 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT stabilizes
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive, DTT stabilizes
-
487104
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE