Information on EC 2.3.1.66 - leucine N-acetyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces roseus

EC NUMBER
COMMENTARY hide
2.3.1.66
-
RECOMMENDED NAME
GeneOntology No.
leucine N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + L-leucine = CoA + N-acetyl-L-leucine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:L-leucine N-acetyltransferase
Propanoyl-CoA can act as a donor, but more slowly. L-Arginine, L-valine, L-phenylalanine and peptides containing L-leucine can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
75496-56-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain MA839-A1, also present in strain MA839-A1 LN-S
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-arginine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + L-leucine
CoA + N-acetyl-L-leucine
show the reaction diagram
-
-
-
?
acetyl-CoA + L-phenylalanine
CoA + N-acetyl-L-phenylalanine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + L-valine
CoA + N-acetyl-L-valine
show the reaction diagram
-
-
-
-
?
propionyl-CoA + L-leucine
CoA + propionyl-L-leucine
show the reaction diagram
-
10% of activity compared to acetyl-CoA
-
-
?
additional information
?
-
-
dipeptides, e.g. L-leucyl-L-leucine, are acetylated more rapidly than L-leucine or peptides with more than two amino acids, D-isomers are acetylated more slowly than L-isomers
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-leucine
CoA + N-acetyl-L-leucine
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
slight stimulation
Fe2+
-
stimulates strongly
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
-
strong
p-chloromercuribenzoate
-
strong
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
acetyl-CoA
-
-
0.06
L-leucine
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
10% of maximal activity at pH 7, maximal activity at pH 9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-180°C, 3 months, 70% remaining activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE