Information on EC 2.3.1.73 - diacylglycerol-sterol O-acyltransferase

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The expected taxonomic range for this enzyme is: Spinacia oleracea

EC NUMBER
COMMENTARY hide
2.3.1.73
-
RECOMMENDED NAME
GeneOntology No.
diacylglycerol-sterol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 1,2-diacyl-sn-glycerol + sterol = a 1-acyl-sn-glycerol + sterol ester
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
1,2-diacyl-sn-glycerol:sterol O-acyltransferase
Cholesterol, sitosterol, campesterol and diacylglycerol can act as acceptors. Transfers a number of long-chain fatty acyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
79586-23-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-diacyl-sn-glycerol + sterol
monoacylglycerol + sterol ester
show the reaction diagram
campesterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + campesterol-3-O-acyl ester
show the reaction diagram
-
-
-
?
cholesterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + cholesterol-3-O-acyl ester
show the reaction diagram
-
-
-
?
diacylglycerol + 1,2-diacyl-sn-glycerol
monoacylglycerol + triacyl-sn-glycerol
show the reaction diagram
-
-
-
?
dipalmitoylglycerol + sterol
monopalmitoylglycerol + steryl palmitate
show the reaction diagram
-
-
-
?
sitosterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + sitosterol-3-O-acyl ester
show the reaction diagram
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Digitonin
-
effective inhibitor
ethanol
-
at 4% inhibits
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
stimulates
-
phosphatidylcholine
-
indispensable for activity
additional information
-
it is possible that Triton X-100 inhibits diacylglycerol degradation and that bovine serum albumin prevents this enzymic inhibition by binding the nonmicellar Triton X-100
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
leaf homogenate
0.0014
-
20000 * g pellet from differential centrifugation of the leaf homogenate
0.0022
-
88000 * g pellet from differential centrifugation of the leaf homogenate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
MES-NaOH buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
differential centrifugation of the leaf homogenate shows the 20000 * g pellet to have the highest amount of enzyme of the fractions tested but the highest specific activity is in the 88000 * g pellet. The active fractions contain endoplasmic reticulum, ribosomes and other membrane vesicles
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Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, in a desiccator, several weeks
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