Information on EC 2.3.1.77 - triacylglycerol-sterol O-acyltransferase

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The expected taxonomic range for this enzyme is: Sinapis alba

EC NUMBER
COMMENTARY hide
2.3.1.77
-
RECOMMENDED NAME
GeneOntology No.
triacylglycerol-sterol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
triacylglycerol + a 3beta-hydroxysteroid = diacylglycerol + a 3beta-hydroxysteroid ester
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
triacylglycerol:3beta-hydroxysterol O-acyltransferase
Tripalmitoylglycerol and, more slowly, other triacylglycerols containing C6 to C22 fatty acids, can act as donors. The best acceptors are 3beta-hydroxysteroids with a planar ring system.
CAS REGISTRY NUMBER
COMMENTARY hide
80487-96-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
white mustard
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
triacylglycerol + cholest-5-en-3beta-ol
diacylglycerols + cholesteryl ester
show the reaction diagram
-
triacylglycerol from olive oil
-
?
trilinoleoylglycerol + cholest-5-en-3beta-ol
dilinoleoylglycerol + cholesteryl linoleate
show the reaction diagram
-
-
-
-
?
trioleoylglycerol + cholest-5-en-3beta-ol
dioleoylglycerol + cholesteryl oleate
show the reaction diagram
-
-
-
-
?
tripalmitoylglycerol + 24R-methylcholest-5-en-3beta-ol
?
show the reaction diagram
-
i.e. campestero l
-
-
?
tripalmitoylglycerol + 5alpha-androstan-3beta-ol
?
show the reaction diagram
-
i.e. androstanol
-
-
?
tripalmitoylglycerol + 5alpha-cholestan-3beta-ol
?
show the reaction diagram
-
i.e. cholestanol, highest rate of esterification
-
-
?
tripalmitoylglycerol + 5beta-cholestan-3beta-ol
?
show the reaction diagram
-
i.e. coprostanol
-
-
?
tripalmitoylglycerol + cholest-5-en-3beta-ol
dipalmitoylglycerol + cholesteryl palmitate
show the reaction diagram
-
i.e.cholesterol
-
?
tripalmitoylglycerol + stigmast-5-en-3beta-ol
?
show the reaction diagram
-
i.e. sitosterol
-
-
?
tripalmitoylglycerol + stigmasta-5,22-dien-3beta-ol
?
show the reaction diagram
-
i.e. stigmasterol
-
-
?
tristearoylglycerol + cholest-5-en-3beta-ol
distearoylglycerol + cholesteryl stearate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the acyltransferase in Sinapis alba roots exhibits group specificity for natural sterols, which are 3beta-hydroxysterols containing a planar ring system and differing only in number or position of double bonds or in the structure of side chain
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
divalent metal ions have no stimulatory effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
reagents containing free sulfhydryl groups e.g. DTT have no effect
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
specific activity in seedlings after various days of germination
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.8
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of seedling
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
gel filtration
additional information
-
two peaks, one more than 100000 Da, the other 18000 Da: the fraction with higher molecular weight may consist of the aggregated form of the enzyme of 18000 Da
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purification has to be carried out at 0-4°C
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, solubilized enzyme preparation, 24 h, 10% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE