Information on EC 2.3.1.90 - beta-glucogallin O-galloyltransferase

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The expected taxonomic range for this enzyme is: Quercus robur

EC NUMBER
COMMENTARY hide
2.3.1.90
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RECOMMENDED NAME
GeneOntology No.
beta-glucogallin O-galloyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 1-O-galloyl-beta-D-glucose = D-glucose + 1-O,6-O-digalloyl-beta-D-glucose
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pentagalloylglucose biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
1-O-galloyl-beta-D-glucose:1-O-galloyl-beta-D-glucose O-galloyltransferase
beta-Glucogallin can act as donor and as acceptor. Digalloylglucose can also act as acceptor, with the formation of 1-O,2-O,6-O-trigalloylglucose
CAS REGISTRY NUMBER
COMMENTARY hide
87502-55-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-O-4-hydroxybenzoyl-beta-D-glucose + 1-O-4-hydroxybenzoyl-beta-D-glucose
1,2-di-O-benzoyl-beta-D-glucose + D-glucose
show the reaction diagram
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-
-
?
1-O-galloyl-beta-D-glucose + 1,6-di-O-galloyl-beta-D-glucose
1,2,6-tri-O-galloyl-beta-D-glucose + D-glucose
show the reaction diagram
-
-
-
?
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
1,6-di-O-galloyl-beta-D-glucose + D-glucose
show the reaction diagram
1-O-protocatechuoyl-beta-D-glucose + 1-O-protocatechuoyl-beta-D-glucose
1,6-di-O-protocatechuoyl-beta-D-glucose + D-glucose
show the reaction diagram
-
-
-
?
1-O-veratroyl-beta-D-glucose + 1-O-veratroyl-beta-D-glucose
1,2-di-O-veratroyl-beta-D-glucose + D-glucose
show the reaction diagram
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-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-O-galloyl-beta-D-glucose + 1-O-galloyl-beta-D-glucose
1,6-di-O-galloyl-beta-D-glucose + D-glucose
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no cofactor requirement
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ion requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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above 50 mM
dithioerythritol
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above 20 mM
additional information
-
no inhibition by metal ions
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
1-O-4-Hydroxybenzoyl-beta-D-glucose
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-
66.7
1-O-Galloyl-beta-D-glucose
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44.4
1-O-Protocatechuoyl-beta-D-glucose
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-
50
1-O-Veratroyl-beta-D-glucose
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-
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0743
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 8
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no activity below pH 4.2 and above pH 8.0
5 - 7.2
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about half-maximal activity at pH 5.0 and pH 7.2
5.5 - 7.5
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about half-maximal activity at pH 5.5 and pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
25% residual activity at 0C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
2-3 months old
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
400000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 6.5
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highest stability at 30C
487707
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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highest stability at pH 4.5-6.5
55 - 60
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denaturation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dilution destabilizes
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glycerol, 10%, stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable in the presence of 10% glycerol
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2-4C, t1/2: 2 days, dilute preparation
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2-4C, t1/2: 7 days, concentrated preparation
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification with ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration
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