Information on EC 2.3.1.B10 - 2-amino-4-ketopentanoate thiolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.B10
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
2-amino-4-ketopentanoate thiolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + D-alanine = CoA + 2-amino-4-oxopentanoate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
urea cycle
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain SB4, specific 2-amino-4-ketopentanoate thiolase activity is about 10% of the activity in extracts of Clostridium sticklandii
-
-
Manually annotated by BRENDA team
strain SB4, specific 2-amino-4-ketopentanoate thiolase activity is about 10% of the activity in extracts of Clostridium sticklandii
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
C1FW07 and C1FW06
catalyzes a step in the ornithine fermentation pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CoA + 2-amino-4-ketopentanoate
acetyl-CoA + D-alanine
show the reaction diagram
CoA + 2-amino-4-oxopentanoate
acetyl-CoA + D-alanine
show the reaction diagram
CoA + 2-amino-4-oxopentanoate + CoA
acetyl-CoA + D-alanine
show the reaction diagram
-
catalyzes a step in the ornithine fermentation pathway
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CoA + 2-amino-4-ketopentanoate
acetyl-CoA + D-alanine
show the reaction diagram
CoA + 2-amino-4-oxopentanoate
acetyl-CoA + D-alanine
show the reaction diagram
C1FW07 and C1FW06
catalyzes a step in the ornithine fermentation pathway
-
-
r
CoA + 2-amino-4-oxopentanoate + CoA
acetyl-CoA + D-alanine
show the reaction diagram
-
catalyzes a step in the ornithine fermentation pathway
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
-
-
pyridoxamine 5'-phosphate
-
-
additional information
-
the enzyme is completely inactivated by dialysis against 100 mM cysteine and can be reactivated by addition of pyridoxal 5'-phosphate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
2-Amino-4-oxopentanoate
C1FW07 and C1FW06
pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate
0.00025 - 0.018
CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4
2-Amino-4-oxopentanoate
Clostridium sticklandii
C1FW07 and C1FW06
pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate
3.5
CoA
Clostridium sticklandii
C1FW07 and C1FW06
pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
97
2-Amino-4-oxopentanoate
Clostridium sticklandii
C1FW07 and C1FW06
pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate
7983
190
CoA
Clostridium sticklandii
C1FW07 and C1FW06
pH 8.0, in presence of 0.04 mM pyridoxal 5'-phosphate
18
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
136000
C1FW07 and C1FW06
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotetramer
C1FW07 and C1FW06
2 * 50000 + 2 * 14000, SDS-PAGE
tetramer
C1FW07 and C1FW06
2 * 13930 + 2 * 51450, calculated from sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24
-
thiolase activity in crude extract remains constant for at least 2 days
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, thiolase activity in crude extract remains constant for at least 10 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
C1FW07 and C1FW06
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as a His-tagged protein
C1FW07 and C1FW06