Information on EC 2.3.2.8 - arginyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.2.8
-
RECOMMENDED NAME
GeneOntology No.
arginyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-arginyl-tRNAArg + protein = tRNAArg + L-arginyl-[protein]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
arginylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arg/N-end rule pathway (eukaryotic)
-
-
SYSTEMATIC NAME
IUBMB Comments
L-arginyl-tRNAArg:protein arginyltransferase
Requires mercaptoethanol and a univalent cation. Peptides and proteins containing an N-terminal glutamate, aspartate or cystine residue can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-24-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
BALB/c
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ecotype Gransden 2004
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-
Manually annotated by BRENDA team
hog
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Arg-tRNA + apolipoprotein A-I
tRNAArg + L-Arg-[apolipoprotein A-I]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + alpha-1-antitrypsin
tRNAArg + L-Arg-[alpha-1-antitrypsin]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + apolipoprotein E
tRNAArg + L-Arg-[apolipoprotein E]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + calreticulin
tRNAArg + L-Arg-[calreticulin]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + contrapsin-like protease inhibitor-3
tRNAArg + L-Arg-[contrapsin-like protease inhibitor-3]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + Cys-beta-galactosidase
tRNaArg + L-Arg-L-Cys-beta-galactosidase
show the reaction diagram
-
substrate only for isoforms Ate1-3, Ate1-4
-
-
?
L-Arg-tRNAArg + glucose-related protein 78
tRNAArg + L-Arg-[glucose-related protein 78]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + hemopexin
tRNAArg + L-Arg-[hemopexin]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + protein-disulfide isomerase
tRNAArg + L-Arg-[protein-disulfide isomerase]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNA + L-Asp-beta-galactosidase
tRNA + L-arginyl-L-Asp-beta-galactosidase
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + acceptor protein
tRNAArg + L-arginyl-[acceptor protein]
show the reaction diagram
L-arginyl-tRNAArg + albumin
tRNAArg + L-arginyl-albumin
show the reaction diagram
L-arginyl-tRNAArg + alpha cardiac actin
tRNAArg + L-arginyl-[alpha cardiac actin]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + alpha lactalbumin
tRNAArg + L-arginyl-[lactalbumin]
show the reaction diagram
L-arginyl-tRNAArg + Asp-beta-galactosidase
tRNAArg + L-arginyl-Asp-beta-galactosidase
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + BiP/GRP78 protein
tRNAArg + L-arginyl-[BiP/GRP78 protein]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + bovine alpha-lactalbumin
tRNAArg + L-arginyl-[bovine alpha-lactalbumin]
show the reaction diagram
-
higher activity is detected with isoforms ATE1-1 (100%) and ATE1-2 (85%), and weaker activity is detected with isoforms ATE1-3 (18%) and ATE1-4 (4%)
-
-
?
L-arginyl-tRNAArg + bovine serum albumin
tRNAArg + L-arginyl-[bovine serum albumin]
show the reaction diagram
L-arginyl-tRNAArg + calreticulin
tRNAArg + L-arginyl-[calreticulin]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + DDIAALVVDNGSGMCK
tRNAArg + ?
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + fructose diphosphatase
tRNAArg + L-Arg-[fructose diphosphatase]
show the reaction diagram
-
from rabbit liver
-
-
?
L-arginyl-tRNAArg + immunoglobulin
tRNAArg + L-arginyl-[immunoglobulin]
show the reaction diagram
-
kappa-light chain of immunoglobulin
-
-
?
L-arginyl-tRNAArg + insulin
tRNAArg + L-arginyl-[insulin]
show the reaction diagram
L-arginyl-tRNAArg + L-Arg-[beta-galactosidase]
tRNAArg + L-Arg-L-Arg-[beta-galactosidase]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + L-Asp-L-Ala
tRNAArg + L-Arg-L-Asp-L-Ala
show the reaction diagram
-
other dipeptides: overview
-
-
?
L-arginyl-tRNAArg + L-aspartic acid
tRNAArg + L-Arg-L-Asp
show the reaction diagram
-
poor substrate
-
?
L-arginyl-tRNAArg + L-cystinyl-bis-L-Ala
tRNAArg + L-Arg-L-cystinyl-bis-L-Ala
show the reaction diagram
-
poor substrate
-
-
?
L-arginyl-tRNAArg + L-Glu-L-Ala
tRNAArg + L-Arg-L-Glu-L-Ala
show the reaction diagram
-
other dipeptides: overview, not dipeptides with D-Glu
-
?
L-arginyl-tRNAArg + L-Glu-L-Ala-L-Ala
tRNAArg + L-Arg-L-Glu-L-Ala-L-Ala
show the reaction diagram
-
other tripeptides: overview
-
-
?
L-arginyl-tRNAArg + L-Glu-[beta-galactosidase]
tRNAArg + L-Arg-L-Glu-[beta-galactosidase]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + L-glutamic acid
tRNAArg + L-Arg-L-Glu
show the reaction diagram
-
poor substrate
-
?
L-arginyl-tRNAArg + L-isoasparagine
tRNAArg + L-arginyl-L-isoasparagine
show the reaction diagram
-
non-peptide-derivatives of dicarboxylic amino acids with blocked alpha-carboxyl group and unsubstituted beta- or gamma-carboxyl group, such as isoasparagine and isoglutamine
-
-
?
L-arginyl-tRNAArg + L-isoglutamine
tRNAArg + L-arginyl-L-isoglutamine
show the reaction diagram
-
non-peptide-derivatives of dicarboxylic amino acids with blocked alpha-carboxyl group and unsubstituted beta- or gamma-carboxyl group, such as isoasparagine and isoglutamine
-
-
?
L-arginyl-tRNAArg + L-Met-[beta-galactosidase]
tRNAArg + L-Arg-L-Met-[beta-galactosidase]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + N-L-aspartyl-N'-dansylamido-1,4-butanediamine
tRNAArg + N-(L-arginyl-L-aspartyl)-N'-dansylamido-1,4-butanediamine
show the reaction diagram
L-arginyl-tRNAArg + protein
tRNAArg + L-arginyl-[protein]
show the reaction diagram
L-arginyl-tRNAArg + protein disulfide isomerase
tRNAArg + L-arginyl-[protein disulfide isomerase]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + RDDIAALVVDNGSGMCK
tRNAArg + ?
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + Rgs16 regulator of G protein
tRNAArg + L-arginyl-[Rgs16 regulator of G protein]
show the reaction diagram
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-
-
-
?
L-arginyl-tRNAArg + Rgs4 regulator of G protein
tRNAArg + L-arginyl-[Rgs4 regulator of G protein]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + Rgs5 regulator of G protein
tRNAArg + L-arginyl-[Rgs5 regulator of G protein]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + thyroglobulin
tRNAArg + L-arginyl-[thyroglobulin]
show the reaction diagram
L-arginyl-tRNAArg + transaldolase
tRNAArg + L-arginyl-[transaldolase]
show the reaction diagram
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I and III from Candida
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-
?
L-arginyl-tRNAArg + trypsin inhibitor
tRNAArg + L-arginyl-[trypsin inhibitor]
show the reaction diagram
-
from soybean
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-
?
L-arginyl-tRNAAsp + beta-actin
tRNAAsp + L-arginyl-[beta-actin]
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Arg-tRNA + apolipoprotein A-I
tRNAArg + L-Arg-[apolipoprotein A-I]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + alpha-1-antitrypsin
tRNAArg + L-Arg-[alpha-1-antitrypsin]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + apolipoprotein E
tRNAArg + L-Arg-[apolipoprotein E]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + calreticulin
tRNAArg + L-Arg-[calreticulin]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + contrapsin-like protease inhibitor-3
tRNAArg + L-Arg-[contrapsin-like protease inhibitor-3]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + glucose-related protein 78
tRNAArg + L-Arg-[glucose-related protein 78]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + hemopexin
tRNAArg + L-Arg-[hemopexin]
show the reaction diagram
-
-
-
-
?
L-Arg-tRNAArg + protein-disulfide isomerase
tRNAArg + L-Arg-[protein-disulfide isomerase]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + acceptor protein
tRNAArg + L-arginyl-[acceptor protein]
show the reaction diagram
L-arginyl-tRNAArg + BiP/GRP78 protein
tRNAArg + L-arginyl-[BiP/GRP78 protein]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + calreticulin
tRNAArg + L-arginyl-[calreticulin]
show the reaction diagram
-
-
-
-
?
L-arginyl-tRNAArg + protein
tRNAArg + L-arginyl-[protein]
show the reaction diagram
L-arginyl-tRNAArg + protein disulfide isomerase
tRNAArg + L-arginyl-[protein disulfide isomerase]
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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the enzyme is independent of ATP
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na+
-
requirement for monovalent cations
NH4+
-
requirement for monovalent cations
additional information
-
inactive with Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aminophenylarsenoxide
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50% inhibition at 0.0025 mM
aurothioglucose
-
complete inhibition at 0.003 mM
Di- and tripeptides
Disulfiram
-
complete inhibition at 0.003 mM
DNAse
-
-
-
Hemin
heparin
isoasparagine
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-
isoglutamine
-
-
L-Glu-L-Val-L-Phe
-
-
merbromin
-
-
N-ethylmaleimide
-
rapid inactivation at 0.2 mM
p-[(bromoacetyl)amino]phenylarsenoxide
-
irreversible, potent inhibitor
phenylarsenoxide
-
inhibition below 0.005 mM is fully reversed by excess dithiothreitol
putrescine
-
above 30 mM
Reactive blue 2
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-
RNase
-
complete inhibition
-
spermidine
spermine
suramin
-
complete inhibition at 0.001 mM
Tannic acid
-
complete inhibition at 0.001 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
dithiothreitol
-
requirement, 0.01 M stabilizes the enzyme in a reduced state
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0005
arginyl-tRNA
-
-
0.03
N-L-aspartyl-N'-dansylamido-1,4-butanediamine
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0667
L-Arginyl-tRNA
Saccharomyces cerevisiae
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
Glu-Val-Phe
-
-
0.0015
heparin
-
-
0.0008
spermine
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-
additional information
additional information
-
overview: di- and tripeptide inhibitors
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00034
merbromin
Mus musculus
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in 50 mM HEPES pH 7.5, 25 mM KCl, 15 mM MgCl2, 0.1 mM dithiothreitol, at 37C
0.00097
Reactive blue 2
Mus musculus
-
in 50 mM HEPES pH 7.5, 25 mM KCl, 15 mM MgCl2, 0.1 mM dithiothreitol, at 37C
0.00043
suramin
Mus musculus
-
in 50 mM HEPES pH 7.5, 25 mM KCl, 15 mM MgCl2, 0.1 mM dithiothreitol, at 37C
0.00028
Tannic acid
Mus musculus
-
in 50 mM HEPES pH 7.5, 25 mM KCl, 15 mM MgCl2, 0.1 mM dithiothreitol, at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.3
-
after addition of inhibitors during purification
7.6
-
-
additional information
-
specific activity depends on type and age of tissue, picomolar range
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 7.8
-
-
8 - 9.8
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about half-maximal activity at pH 8.0, about 80% of maximal activity at pH 9.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
embryonic
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
ATE1-1p and ATE1-2p
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
-
SDS-PAGE
60000
-
determined by SDS-PAGE and Western Blot analysis
360000
-
multienzyme complex of several molecules of arginyl-tRNA synthetase and arginyl-tRNA-protein transferase, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol or dithiothreitol stabilize during storage
-
freezing inactivates
-
glycerol, 20%, stabilizes concentrated enzyme solutions during storage
-
phosphate, 0.04 M, stabilizes
-
polyethylene glycol, 30%, stabilizes concentrated solutions during storage
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, crude enzyme, at least 6 months
-
0C, concentrated enzyme solution, 20% glycerol, t1/2: about 1 week
-
0C, partially purified, 75% loss of activity within 1 week
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4C, concentrated enzyme solution, 0.5 M phosphate-KOH-buffer, pH 7.8, 0.1 M 2-mercaptoethanol, up to 6 months
-
4C, purified, 0.05 M Tris-HCl buffer, pH 8.0, 0.1 M 2-mercaptoethanol, 0.09 M KCl, 0.02 M spermine, 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on heparin-Sepharose and angiotensin II-Sepharose combined with affinity elution with lactalbumin
-
affinity chromatography on heparin-Sepharose, homogeneity
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Mono-S column chromatography
-
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 species: Ate1-1 Ate1-2, fusion protein with green fluorescent protein
-
a targeting vector is constructed to generate Ate1-deficient mice
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expressed in Escherichia coli BL21(DE3) cells
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mouse genomic DNA encoding Ate1 is isolated from a BAC library, Ate1-deficient mouse strains are constructed
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is 2fold induced after 3 days and 7-8fold after 7 days of auxin application
-
enzyme expression is not induced by abscisic acid or cytokinin
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the enzyme is downregulated in several types of human cancer samples at the protein level
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F313A
-
slight decrease in activity
K417A
-
no detectable enzymatic activity
P370A
-
increase in activity, especially on Asp-containing substrate
Y416A
-
no effect on Asp-containing substrate, but affects Glu-containing substrate
C315A
-
full enzyme activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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arginyltransferase inversely correlates with metastases in human cancers and patient survival
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