Information on EC 2.3.2.B13 - ubiquitin transferase RING E3 (cullin-dependent)

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The expected taxonomic range for this enzyme is: Homo sapiens

EC NUMBER
COMMENTARY hide
2.3.2.B13
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
ubiquitin transferase RING E3 (cullin-dependent)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine = [cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
[cullin-RING-E3-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine:acceptor protein ubiquitin transferase (isopeptide bond-forming)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine
[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-ubiquitinyl-[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-L-lysine
[cullin-RING-E3-ubiquitin-carrier protein]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-terminal domain in complex with von Hippel-Lindau tumor suppressor protein, elonhin B, and elongin C, hanging drop vapor diffusion method, using 5 M sodium formate as precipitant
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, glutatione S-transferase column chromatography, maltose binding protein-Trap column chromatography, anion exchange chromatorgaphy, and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells