Information on EC 2.3.2.B14 - L,D-transpeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.2.B14
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
L,D-transpeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala4 bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap3 of the donor stem and the side chain of mDap3 of the acceptor stem.
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptidyl group transfer
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catalyzes the formation of 3-3 cross-links between two peptidoglycan subunits, the third position of the stem peptide consists of a D-Asp or a D-Asn residue (D-Asx) linked to the beta-amine of L-Lys via the beta-carboxyl, the 3-3 cross-link connects the beta-carboxyl of L-Lys in the donor to the alpha amine of D-Asx in the acceptor
SYSTEMATIC NAME
IUBMB Comments
peptidoglycan (3->3)-transpepdidase
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(L-Lys)3-D-iso-Asn + (L-Lys)3-L-Ala-L-Ala
?
show the reaction diagram
(L-Lys)3-L-Ala-L-Ala + ?
?
show the reaction diagram
2 GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala 3->3 crosslinked at the mesoDapNH2 position
show the reaction diagram
AcPhe-tRNA + puromycin
tRNA + AcPhe-puromycin
show the reaction diagram
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?
meso-diaminopimelic acid + (L-Lys)3-D-iso-Asn
?
show the reaction diagram
meso-diaminopimelic acid + (L-Lys)3-L-Ala-L-Ala
?
show the reaction diagram
peptidyl-tRNA1 + alpha-aminoacyl-tRNA2
tRNA1 + peptidyl-amino-tRNA2
show the reaction diagram
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?
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
carbapenem beta-32
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doripenem
ertapenem
Imipenem
meropenem
additional information
not inhibited by clavulanic acid
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
pH optimum for beta-lactamase activity with nitrocefin
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapour diffusion method
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hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), 1 M succinic acid, and 1% (w/v) PEG MME 2000
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM Tris-HCl pH 7.5 containing 100 mM NaCl
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-80 °C, 50 mM Tris-HCl pH 7.5 containing 300 mM NaCl
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity anion exchange chromatography and gel filtration
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Ni-NTA agarose resin column chromatography and Superdex 75 gel filtration
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Ni-NTA agarose resin column chromatography, MonoQ column chromatography, and Superdex HR10/30 gel filtration
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Ni2+ nitrilotriacetate-agarose resin affinity chromatography
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Ni2+ nitrilotriacetate-agarose resin affinity chromatography, MonoQ HR5/5 chromatography, and Superdex HR10/30 column gel filtration
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nickel affinity column chromatography and Sepharose 12 column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
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expressed in Mycobacterium bovis strain Bacille Calmette-Guerin
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the extramembrane portion of the L,D-transpeptidase is expressed in Escherichia coli BL21(DE3) cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE