Information on EC 2.4.1.118 - cytokinin 7-beta-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.118
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RECOMMENDED NAME
GeneOntology No.
cytokinin 7-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + an N6-alkylaminopurine = UDP + an N6-alkylaminopurine-7-beta-D-glucoside
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:N6-alkylaminopurine 7-glucosyltransferase
Acts on a range of N6-substituted adenines, including zeatin and N6-benzylaminopurine, but not N6-benzyladenine. With some acceptors, 9-beta-D-glucosides are also formed.
CAS REGISTRY NUMBER
COMMENTARY hide
72103-03-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dihydrozeatin + TDP-glucose
dihydrozeatin 7-beta-D-glucoside + TDP
show the reaction diagram
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-
-
?
kinetin + TDP-glucose
kinetin 7-beta-D-glucoside + TDP
show the reaction diagram
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-
-
?
N6-benzyladenine + TDP-glucose
N6-benzyladenine 7-beta-D-glucoside + TDP
show the reaction diagram
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-
-
?
N6-isopentenyladenine + TDP-glucose
N6-isopentenyladenine 7-beta-D-glucoside + TDP
show the reaction diagram
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-
-
?
TDP-glucose + zeatin
TDP + zeatin 7-beta-D-glucoside
show the reaction diagram
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as effective as UDPglucose
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-
?
trans-zeatin + TDP-glucose
trans-zeatin 7-beta-D-glucoside + TDP
show the reaction diagram
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-
-
?
UDPglucose + 6-benzylaminopurine
UDP + 6-benzylaminopurine-7-beta-D-glucoside
show the reaction diagram
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activity is 15% higher for 6-benzylaminopurine than for zeatin
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-
?
UDPglucose + dihydroxyzeatin
UDP + dihydroxyzeatin 7-beta-D-glucoside
show the reaction diagram
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-
-
-
?
UDPglucose + N6-alkylaminopurine
UDP + N6-alkylaminopurine-7-beta-D-glucoside
show the reaction diagram
UDPglucose + zeatin
UDP + zeatin 7-beta-D-glucoside
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + N6-alkylaminopurine
UDP + N6-alkylaminopurine-7-beta-D-glucoside
show the reaction diagram
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involved in regulation of cytokinin activity
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-methyl-3-(2-methylpropyl)-3,7-dihydro-1H-purine-2,6-dione
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2-Chloro-6-(5-hydroxypentylamino)-7-methylpurine
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2-Chloro-6-(5-hydroxypentylamino)-9-methylpurine
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3-isobutyl-1-methylxanthine
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3-Methyl-7-pentylaminopyrazolo[4,3-d]pyrimidine
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competitive
4-Cyclopentylamino-2-methylthiopyrrolo[2,3-d]pyrimidine
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4-Furfurylaminopyrazolo[3,4-d]pyrimidine
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6-(5-Hydroxypentylamino)-9-methylpurine
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6-Benzylamino-2-(2-hydroxyethylamino)-7-methylpurine
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6-Benzylamino-2-(2-hydroxyethylamino)-9-methylpurine
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6-Benzylamino-9-(3-hydroxyprolyl)purine
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N-(3-Chlorophenyl)-N'-phenylurea
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N-Benzyl-N'-phenylurea
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theophylline
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16 - 0.22
dihydrozeatin
0.07 - 0.21
kinetin
0.04 - 0.09
N6-benzyladenine
0.07 - 0.13
N6-isopentenyladenine
0.22 - 0.24
trans-zeatin
0.19
UDPglucose
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0.15
zeatin
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.22
2-Chloro-6-(5-hydroxypentylamino)-7-methylpurine
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0.048
2-Chloro-6-(5-hydroxypentylamino)-9-methylpurine
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0.022
3-Methyl-7-pentylaminopyrazolo[4,3-d]pyrimidine
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0.027
4-Cyclopentylamino-2-methylthiopyrrolo[2,3-d]pyrimidine
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0.28
4-Furfurylaminopyrazolo[3,4-d]pyrimidine
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0.23
6-(5-Hydroxypentylamino)-9-methylpurine
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0.061
6-Benzylamino-2-(2-hydroxyethylamino)-7-methylpurine
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0.0033
6-Benzylamino-2-(2-hydroxyethylamino)-9-methylpurine
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0.2
6-Benzylamino-9-(3-hydroxyprolyl)purine
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0.075
N-(3-Chlorophenyl)-N'-phenylurea
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0.1
N-Benzyl-N'-phenylurea
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0.0016 - 0.0025
Olomoucine
0.38
theophylline
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00046
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 7.4
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46500
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE