Information on EC 2.4.1.136 - gallate 1-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Quercus rubra

EC NUMBER
COMMENTARY hide
2.4.1.136
-
RECOMMENDED NAME
GeneOntology No.
gallate 1-beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + gallate = UDP + 1-galloyl-beta-D-glucose
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
galloylated catechin biosynthesis
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pentagalloylglucose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:gallate beta-D-glucosyltransferase
A number of substituted benzoic acids and, more slowly, cinnamic acids, can act as acceptors. Vanillin is the best acceptor investigated.
CAS REGISTRY NUMBER
COMMENTARY hide
89700-30-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + cinnamate
UDP + 1-O-cinnamoyl-beta-D-glucose
show the reaction diagram
-
9% of activity compared to vanillate
-
-
?
UDP-glucose + p-coumarate
UDP + 1-O-p-coumaroyl-beta-D-glucose
show the reaction diagram
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32% of activity compared to vanillate
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-
?
UDPglucose + 3,4,5-trimethoxybenzoate
UDP + 1-O-3,4,5-trimethoxybenzoyl-beta-D-glucose
show the reaction diagram
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7% of activity compared to vanillate
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-
?
UDPglucose + anisate
UDP + 1-O-anisoyl-beta-D-glucose
show the reaction diagram
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38% of activity compared to vanillate
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-
?
UDPglucose + benzoate
UDP + benzoyl-beta-D-glucose
show the reaction diagram
-
16% of activity compared to vanillate
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-
?
UDPglucose + ferulate
UDP + 1-O-feruloyl-beta-D-glucose
show the reaction diagram
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35% of activity compared to vanillate
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-
?
UDPglucose + gallate
UDP + 1-O-galloyl-beta-D-glucose
show the reaction diagram
UDPglucose + m-coumarate
UDP + 1-O-m-coumaroyl-beta-D-glucose
show the reaction diagram
-
14% of activity compared to vanillate
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-
?
UDPglucose + p-hydroxybenzoate
UDP + 1-O-hydroxybenzoyl-beta-D-glucose
show the reaction diagram
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48% of activity compared to vanillate
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-
?
UDPglucose + protocatechuate
UDP + protocatechuyl-beta-D-glucose
show the reaction diagram
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22% of activity compared to vanillate
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-
?
UDPglucose + sinapate
UDP + 1-O-sinapoyl-beta-D-glucose
show the reaction diagram
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13% of activity compared to vanillate
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-
?
UDPglucose + vanillate
UDP + vanilloyl-beta-D-glucose
show the reaction diagram
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best substrate
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-
?
UDPglucose + veratrate
UDP + 1-O-veratroyl-beta-D-glucose
show the reaction diagram
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71% of activity compared to vanillate
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + gallate
UDP + 1-O-galloyl-beta-D-glucose
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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4 mM, slight
UDP
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strong
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1 - 1.11
gallate
3.45
p-hydroxybenzoate
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2.3
UDPglucose
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reaction with gallate
0.57
vanillate
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0.72
veratrate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0117
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reaction with gallate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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7
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Tris-HCl or KHPO4- buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7 - 9
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50% of maximal activity at pH 4.7 and 9.0
5.5 - 8.5
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50% of maximal activity at pH 5.5 and 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
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gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol in extraction buffers stabilizes
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diluted enzyme is unstable
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive, mercaptoethanol stabilizes
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488579
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, 1-2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE