Information on EC 2.4.1.140 - alternansucrase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.140
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RECOMMENDED NAME
GeneOntology No.
alternansucrase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Transfers alternately an alpha-D-glucosyl residue from sucrose to the 6-position and the 3-position of the non-reducing terminal residue of an alpha-D-glucan, thus producing a glucan having alternating alpha-(1->6)- and alpha-(1->3)-linkages
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
sucrose:(1->6)[(1->3)]-alpha-D-glucan 6(3)-alpha-D-glucosyltransferase
The product, which has quite different properties from other dextrans, has been called alternan.
CAS REGISTRY NUMBER
COMMENTARY hide
100630-46-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
fragment
UniProt
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
stevioside + sucrose
?
show the reaction diagram
sucrose + alpha-D-glucan
alternating-1,6-1,3-alpha-D-glucan
show the reaction diagram
sucrose + alpha-D-glucopyranosyl-(1,4)-L-glucose
?
show the reaction diagram
sucrose + butyl alpha-D-glucopyranoside
?
show the reaction diagram
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-
-
-
?
sucrose + cellobiose
oligosaccharides
show the reaction diagram
sucrose + D-tagatose
alpha-D-glucopyranosyl-(1,1)-beta-D-tagatopyranose
show the reaction diagram
sucrose + gentiobiose
?
show the reaction diagram
sucrose + isomaltose
?
show the reaction diagram
sucrose + isomaltose
oligoalternan
show the reaction diagram
sucrose + L-glucose
alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,4)-L-glucose
show the reaction diagram
sucrose + leucrose
?
show the reaction diagram
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regioselectivity of alternansucrase differs from dextransucrase. Alternansucrase shows greater ability to use leucrose as an acceptor, alternansucrase continues to transfer glucosyl units to leucrose, resulting in some unusual glucosyl-fructose oligosaccharides
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?
sucrose + luteolin
luteolin-3'-O-alpha-D-glucopyranoside + luteolin-4'-O-alpha-D-glucopyranoside
show the reaction diagram
sucrose + maltose
?
show the reaction diagram
sucrose + maltose
oligoalternan
show the reaction diagram
sucrose + methyl alpha-D-allo-pyranoside
methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside + alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside + beta-D-fructofuranose
show the reaction diagram
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the product methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside is subsequently glucosylated at position 6 to give rise to the trisaccharide methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-glucopyranosyl-(1,6)-alpha-D-allopyranoside. Higher DP products are observed
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?
sucrose + methyl alpha-D-galactopyranoside
methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside + methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside + beta-D-fructofuranose
show the reaction diagram
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two initial products methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside and methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside, in a 2.5:1 molar ratio
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?
sucrose + methyl alpha-D-galactopyranoside
methyl alpha-D-glucopyranosyl-(1,4)-alpha-D-galactopyranoside + methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside + beta-D-fructofuranose
show the reaction diagram
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production of methyl alpha-D-glucopyranosyl-(1, 4)-alpha-D-galactopyranoside and methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-galactopyranoside in the ratio 2.5:1
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?
sucrose + methyl alpha-D-mannopyranoside
methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-mannopyranoside + methyl-3,6-di-O-alpha-D-glucopyranosyl-alpha-D-mannopyranoside + beta-D-fructofuranose
show the reaction diagram
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the major initial acceptor product is methyl alpha-D-glucopyranosyl-(1,6)-alpha-D-mannopyranoside, but several minor products are also isolated and characterized, including a 3,6-di-O-substituted mannopyranoside
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?
sucrose + methyl beta-D-galactopyranoside
?
show the reaction diagram
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poor substrate
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?
sucrose + methyl beta-D-mannopyranoside
?
show the reaction diagram
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poor substrate
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?
sucrose + methyl-alpha-D-glucoside
oligoalternan
show the reaction diagram
sucrose + methyl-beta-D-glucopyranoside
methyl beta-isomaltoside + methyl beta-isomaltotrioside + methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranoside + beta-D-fructofuranose
show the reaction diagram
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the initial product arising is methyl beta-isomaltoside, which is subsequently glucosylated to yield methyl beta-isomaltotrioside and methyl alpha-D-glucopyranosyl-(1,3)-alpha-D-glucopyranosyl-(1,6)-beta-D-glucopyranoside
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?
sucrose + myricetin
?
show the reaction diagram
sucrose + octyl-alpha-D-glucopyranoside
?
show the reaction diagram
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-
-
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?
sucrose + quercetin
?
show the reaction diagram
sucrose + raffinose
?
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Aminoethanol
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3-Deoxy-3-fluoro-alpha-D-glucopyranosyl fluoride
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octyl beta-D-glucopyranoside
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Tris(hydroxymethyl)aminomethane
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additional information
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below a sodium phosphate buffer concentration of 50 mM the activity is reduced by 75%
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Dextran T10
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1.7fold stimulation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.9 - 16.3
Sucrose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
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activity profile, overview
4 - 7.2
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pH 4.0: about 75% of maximal activity, pH 7.2: about 60% of maximal activity
4.6 - 6.4
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about 50% of activity maximum at pH 4.6 and 6.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
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activity profile, overview
30 - 50
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30°C: about 65% of maximal activity, 50°C: about 55% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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the concentration of the enzyme in the particulate fraction suggests that it is associated primarily with the cell wall or membrane
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Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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1.5% carbohydrate
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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pH 5.4, half-life: 2 days
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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expression in Solanum tuberosum. The Asr transcript levels correlated well with alternan accumulation in tuber juices
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recombinant expression of the large alternansucrase (2057 amino acids) is hindered in Escherichia coli due to poor enzyme solubility and protein degradation. A truncated variant deleted of the APY repeats but harboring four C-terminal CW-like repeats display a high specific activity and the same specificity of product synthesis as the native enzyme. It is more soluble and suffers less degradation than full length alternansucrase
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
synthesis
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modification of bacterial cellulose using sucrose, dextransucrase from Leuconostoc mesenteroides B-742CMB and alternansucrase from Leuconostoc B-1355C. This modification method produces a new bacterial cellulose that has a unique structure and probably new property
additional information