Information on EC 2.4.1.145 - alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.145
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RECOMMENDED NAME
GeneOntology No.
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannosyl-glycoprotein N-acetylglucosaminyltransferases
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N-Glycan biosynthesis
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Various types of N-glycan biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:3-[2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl]-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor (click here for diagram). The best acceptor for this enzyme is probably the same as that favoured by EC 2.4.1.144, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
86498-16-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + biantennary N-glycan
?
show the reaction diagram
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the enzyme transfers a N-acetyl-D-glucosamine from UDP-N-acetyl-D-glucosamine to the biantennary oligosaccharide and produces triantennary N-glycans with a beta1,4-N-acetyl-D-glucosamine on the alpha1,3-Man arm
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-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2(GlcNAcbeta1-6)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
UDP + ?
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
?
show the reaction diagram
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isozyme GnT-IVb has about 30% activity of isozyme GnT-IVa
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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Mg2+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
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EDTA
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Mn2+
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at high concentration
UDP
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additional information
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sugar nucleotides having the UDP moiety inhibit activity to different extent
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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maximum activation at 0.125%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.532 - 3.35
GlcNAcbeta1-2(GlcNAcbeta1-6)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
1.04 - 6.94
GlcNAcbeta1-2Man1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
0.971 - 5.72
GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
3.19 - 10.5
GlcNAcbeta1-2Manalpha1-3(Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine
3.4
pyridylaminated acceptor substrate
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0.118 - 0.358
UDP-N-acetyl-D-glucosamine
8.3
UDP-N-acetylglucosamine
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pyridylaminated acceptor substrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.65
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additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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in presence of 0.625% Triton X-100
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
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depends on Triton X-100 concentration
7 - 8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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low activity
Manually annotated by BRENDA team
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high activity
Manually annotated by BRENDA team
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high activity
Manually annotated by BRENDA team
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low activity
Manually annotated by BRENDA team
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isoform GnT-IVa is highly restricted in gastrointestinal organs and peripheral blood leukocytes
Manually annotated by BRENDA team
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low activity
Manually annotated by BRENDA team
high expression of GnT-IVa, profiles and structures of the cell-surface N-glycan of hepatocellular carcinoma cells, high levels of NA3Fb, a core-alpha-1,6-fucosylated triantennary glycan, overview
Manually annotated by BRENDA team
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in epirubicin-resistant HLE cells, GnT-IVa expression is decreased; in epirubicin-resistant HLE cells, GnT-IVb expression is increased
Manually annotated by BRENDA team
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low activity
Manually annotated by BRENDA team
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low activity
Manually annotated by BRENDA team
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; low activity
Manually annotated by BRENDA team
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isoform GnT-IVa is highly restricted in gastrointestinal organs and peripheral blood leukocytes
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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high activity
Manually annotated by BRENDA team
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Panc-1, PCI6, MIA PaCa-2, and BxPC3. Down-regulation of GnT-IVa in pancreatic cancer cells is due to an epigenetic abnormality in the gene. When pancreatic cancer cells are treated with 5-aza-C or butyrate, the expression of GnT-IVa is dramatically increased; Panc-1, PCI6, MIA PaCa-2, and BxPC3. Expression of GnT-IVb is increased in pancreatic cancer, compared to normal pancreatic tissues
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
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deduced from SDS-PAGE and gel filtration results
61610
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deduced from cDNA sequence
63200
isozyme b, deduced from cDNA sequence
66000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 58000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Asn-linked sugar chains
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, crude microsomal preparation, several months in detergent-free buffer
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
IgG Sepharose resin column chromatography
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Q-Sepharose and Cu2+ Sepharose chromatography, followed by UDP-hexanolamine-agarose affinity chomatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana
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expressed in Spodoptera frugiperda and Trichoplusia ni cells by using a baculovirus expression vector
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expression in COS-7 cells
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expression in COS7 cells
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expression of the isozyme b in COS7 cells
gene MGAT4A, expression analysis
recombinant full-length enzyme and soluble truncated form tagged with FLAG sequence at the N-terminus. expressed in COS7 cells, N-acetylglucosaminyltransferase-IVa; recombinant full-length enzyme and soluble truncated form tagged with FLAG sequence at the N-terminus. expressed in COS7 cells, N-acetylglucosaminyltransferase-IVb
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Mgat4a expression is induced under hypoxic conditions. Isoform GnT-IVa expression is aberrantly up-regulated in some type of tumor cells
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Mgat4a expression levels are significantly reduced in the pancreatic beta cells of type 2 diabetes patients
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
knockdown of GnT-IVa in 1668 cells and HepG2 cells by transfection with GnT-IVa shRNA. The level of NA3Fb decreases and M8 increases in knockdown GnT-IVa/1668 cells compared with GnT-IVa/NC after 48 h transfection. The scratch healing rates of the GnT-IVa/1668 group decreases more than the GnT-IVa/NC group. High expression of GnT-IVa leads to a higher level of NA3Fb in the HCC cell surface, and the downregulation of GnT-IVa may modulate cell invasion and migration through NA3Fb glycan
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
synthesis
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it is necessary to select appropriate host cell lines according to their glycosyltransferases activity balance to produce glycoproteins with an objective sugar chain structure. The descibed method of grouping cell lines according to their glycosyltransferases activity balance is useful to select appropriate host cells