Information on EC 2.4.1.187 - N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.187
-
RECOMMENDED NAME
GeneOntology No.
N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
poly(3-O-beta-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis
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poly(glycerol phosphate) wall teichoic acid biosynthesis
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poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis)
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poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus)
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teichoic acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-mannosamine:N-acetyl-beta-D-glucosaminyldiphosphoundecaprenol 4-beta-N-acetylmannosaminyltransferase
Involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls.
CAS REGISTRY NUMBER
COMMENTARY hide
118731-82-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AHU 1035
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
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0.3-0.5 M: stimulation
KCl
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0.3 M: stimulation
MgCl2
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10 mM, highest stimulatory effect
NH4Cl
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0.3-0.5 M: stimulation
additional information
-
activity does not improve with the addition of Mg2+ at concentrations up to 30 mM; activity does not improve with the addition of Mn2+ at concentrations up to 30 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-mannopyranosyl]-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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is an I-parabolic, S-parabolic noncompetitive inhibitor with respect ro UDP-ManNAc and an I-linear, S-parabolic noncompetitive inhibitor with respect to the acceptor 3 at nonsaturating levels
Triton X-100
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strong
UDP
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2 mM, strong
additional information
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activity does not decrease with the addition of EDTA
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerol
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25%: twofold increase of activity
Nonidet P-40
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0.1%: small stimulatory effect
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.193
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.03
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.022
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.035
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.258
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.0044
UDP-N-acetylmannosamine
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 9.183
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
10.25
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
Bacillus subtilis
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apparent Kcat at 600 microM UDP-ManNAc
0.52 - 13.92
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
0.04 - 8.617
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
0.866 - 6.08
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000022
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
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TagA shows optimal acitvity at pH 7.8 in Bis-Tris propane buffer. Tested at room temperatur
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
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40% of maximal activity at pH 6, 45% of maximal activity at pH 9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 25%, stabilizes
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Nonidet P-40, 0.1-0.2%, stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, several months, no significant loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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