Information on EC 2.4.1.190 - luteolin-7-O-glucuronide 2''-O-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY hide
2.4.1.190
-
RECOMMENDED NAME
GeneOntology No.
luteolin-7-O-glucuronide 2''-O-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucuronate + luteolin 7-O-beta-D-glucuronide = UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
luteolin triglucuronide biosynthesis
-
-
Flavone and flavonol biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucuronate:luteolin-7-O-beta-D-glucuronide 2''-O-glucuronosyltransferase
The enzyme participates in the biosynthesis of luteolin triglucuronide, the major flavone found in the photosynthetically-active mesophyll of the primary leaves of Secale cereale (rye).
CAS REGISTRY NUMBER
COMMENTARY hide
115490-51-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucuronic acid + apigenin 7-O-beta-D-glucuronide
UDP + apigenin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
show the reaction diagram
-
88% of activity with luteolin 7-O-glucuronide
-
?
UDP-glucuronic acid + chrysoeriol 7-O-beta-D-glucuronide
UDP + chrysoeriol 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
show the reaction diagram
-
65% of activity with luteolin-7-O-glucuronide
-
-
?
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
show the reaction diagram
UDPglucuronic acid + apigenin 7-O-glucoside
UDP + apigenin-7-O-beta-D-glucosyl-1,4-beta-D-glucuronoside
show the reaction diagram
-
51.5% of activity with luteolin 7-O-glucuronide
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
show the reaction diagram
-
involved in the sequential biosynthesis of luteolin triglucuronide
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
0.75 mM, 40% inhibition
Cu2+
-
0.75 mM, 40% inhibition
luteolin
-
0.1 mM, 50% inhibition
Pb2+
-
0.75 mM, 40% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
luteolin 7-O-glucuronide
-
-
0.04
UDPglucuronic acid
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
UDP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
pH-optimum at pH 8.5 is absent in crude enzyme preparations
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
50% of maximal activity at pH 6.0 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
no activity in vacuoles
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
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gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing without glycerol, complete inactivation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ultrogel AcA 44, ultrafiltration, hydroxylapatite, partial purification
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