Information on EC 2.4.1.192 - nuatigenin 3beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Avena sativa

EC NUMBER
COMMENTARY hide
2.4.1.192
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RECOMMENDED NAME
GeneOntology No.
nuatigenin 3beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol = UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:(20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucosyltransferase
Some other sapogenins can act as glucosyl acceptors. Involved in the biosynthesis of plant saponins. Not identical with EC 2.4.1.173 (sterol 3beta-glucosyltransferase) or EC 2.4.1.193 (sarsapogenin 3beta-glucosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
108891-57-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose
UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
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i.e. tigogenin, at 12% of the activity with nuatigenin
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-
?
(25R)-5alpha-spirostane-3beta,6alpha-diol + UDP-glucose
UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside
show the reaction diagram
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i.e. chlorogenin, at 30% of the activity with nuatigenin
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-
?
(25R)-spirost-5-en-3beta-ol + UDP-glucose
UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
20S,22S,25S-spirost-5-ene-3beta,25-diol + UDP-glucose
UDP + 20S,22S,25S-spirost-5-ene-3beta,25-diol 3-O-beta-D-glucoside
show the reaction diagram
22S,25S-5alpha-tomatanin-3beta-ol + UDP-glucose
UDP + 22S,25S-5alpha-tomatanin-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
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i.e. tomatidine, at 57% of the activity with nuatigenin
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-
?
22S,25S-solanid-5-enin-3beta-ol + UDP-glucose
UDP + gamma-chaconine
show the reaction diagram
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i.e. solanidine, at 39% of the activity with nuatigenin
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?
androst-5-en-3beta-ol-17-one + UDP-glucose
UDP + androst-5-en-3beta-ol-17-one 3-O-beta-D-glucoside
show the reaction diagram
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i.e. androsterone, at 15% of the activity with nuatigenin
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pregn-5-en-3beta-ol-20-one + UDP-glucose
UDP + pregn-5-en-3beta-ol-20-one 3-O-beta-D-glucoside
show the reaction diagram
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i.e. pregnenolone, at 26% of the activity with nuatigenin
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?
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol
UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol
UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside
show the reaction diagram
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participates in the initiation of sugar chain formation during the biosynthesis of oat saponins: avenacosides A and B
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no stimulation by divalent cations: Ca2+, Mn2+ or Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-chloromercuribenzoate
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Triton X-100
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
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7.5 - 8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.5
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at pH 5.8 about 50% of activity maximum, at pH 6.5-8.5 activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 39
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at 30C activity maximum, at 39C activity is 7times lower than at 30C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000 - 62000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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unstable
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol, 10 mM, improves stability in solution, 35% loss of activity after 24 h at 4C
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rather unstable when dissolved in buffer, 65% loss of activity after 24 h at 4C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, cytosolic fraction stable for several months
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-20C, in the form of dry acetone powder, stable for several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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