Information on EC 2.4.1.193 - sarsapogenin 3beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Asparagus officinalis

EC NUMBER
COMMENTARY hide
2.4.1.193
-
RECOMMENDED NAME
GeneOntology No.
sarsapogenin 3beta-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + (25S)-5beta-spirostan-3beta-ol = UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:(25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucosyltransferase
Specific to 5beta-spirostanols. Involved in the biosynthesis of plant saponins. Not identical with EC 2.4.1.173 (sterol 3beta-glucosyltransferase) or EC 2.4.1.192 (nuatigenin 3beta-glucosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
117698-14-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPglucose + (25R)-5alpha-spirostan-3beta-ol
UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
UDPglucose + (25R)-5alpha-spirostan-3beta-ol-12-one
UDP + (25R)-5alpha-spirostan-3beta-ol-12-one 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
UDPglucose + (25R)-5alpha-spirostane-3beta,6alpha-diol
UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
UDPglucose + (25R)-5beta-spirostan-3beta-ol
UDP + (25R)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
i.e. smilagenin
-
-
?
UDPglucose + (25R)-spirost-5-en-3beta-ol
UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
UDPglucose + (25S)-5beta-spirostan-3beta-ol
UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
UDPglucose + 5alpha-cholestan-3beta-ol
UDP + 5alpha-cholestan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
UDPglucose + 5beta-cholestan-3beta-ol
UDP + 5beta-cholestan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
UDPglucose + stigmast-5-en-3beta-ol
UDP + stigmast-5-en-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
weak activity
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucose + (25S)-5beta-spirostan-3beta-ol
UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
show the reaction diagram
-
involved in the biosynthesis of plant saponins
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
1 mM, stimulates
Mn2+
-
1 mM, stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
1 mM, 43% inhibition
EGTA
-
10 mM, 50% inhibition
PCMB
-
0.01 mM, 94% inhibition
Triton X-100
-
-
UDP
-
1 mM, 89% inhibition
UMP
-
1 mM, 51% inhibition
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 9.9
-
pH 7.2: about 40% of maximal activity, pH 9.9: about 60% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of 3-week-old plants
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE