Information on EC 2.4.1.2 - dextrin dextranase

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The expected taxonomic range for this enzyme is: Gluconobacter oxydans

EC NUMBER
COMMENTARY hide
2.4.1.2
-
RECOMMENDED NAME
GeneOntology No.
dextrin dextranase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-alpha-D-glucan:(1->6)-alpha-D-glucan 6-alpha-D-glucosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9032-13-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m
(1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
show the reaction diagram
1,4-alpha-D-amyloheptaose + (1,6-alpha-D-glucopyranosyl)n
dextran
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucopyranose
show the reaction diagram
cellobiose + starch
?
show the reaction diagram
-
starch is the donor substrate, cellobiose the acceptor substrate
-
-
?
D-glucosyl-alpha-(1,4)-D-xylose + starch
?
show the reaction diagram
-
starch is the donor substrate, D-glucosyl-alpha(1,4)-D-xylose the acceptor substrate
-
-
?
dextran + salicin
?
show the reaction diagram
-
dextran is the donor substrate, salicin the acceptor substrate
-
-
?
dextran + salicin
glucosyl-alpha-1,6-salicin + isomaltosyl-alpha-1,6-salicin
show the reaction diagram
-
-
-
?
gentiobiose + starch
?
show the reaction diagram
-
starch is the donor substrate, gentiobiose the acceptor substrate
-
-
?
isomaltooligosaccharide
glucose + isomaltooligosaccharides
show the reaction diagram
-
-
-
?
isomaltose
dextran
show the reaction diagram
-
-
-
?
isomaltose + salicin
?
show the reaction diagram
-
isomaltose is the donor substrate, salicin the acceptor substrate
-
-
?
isomaltose + salicin
glucosyl-alpha-1,6-salicin + isomaltosyl-alpha-1,6-salicin
show the reaction diagram
-
-
-
?
isomaltose + starch
?
show the reaction diagram
-
starch is the donor substrate, isomaltose the acceptor substrate
-
-
?
isoprimeverose + starch
?
show the reaction diagram
-
starch is the donor substrate, isoprimeverose the acceptor substrate
-
-
?
kojibiose + starch
?
show the reaction diagram
-
starch is the donor substrate, kojibiose the acceptor substrate
-
-
?
laminaribiose + starch
?
show the reaction diagram
-
starch is the donor substrate, laminaribiose the acceptor substrate
-
-
?
maltohexaose
dextran
show the reaction diagram
maltooligosaccharide
alpha-1,6-glucan
show the reaction diagram
-
-
-
?
maltooligosaccharide
dextran
show the reaction diagram
-
-
-
?
maltopentaose
dextran
show the reaction diagram
maltose
dextran
show the reaction diagram
-
no activity with kojibiose, sophorose, nigerose, laminaribiose, cellobiose, gentiobiose or trehalose
-
?
maltose + salicin
?
show the reaction diagram
-
maltose is the donor substrate, salicin the acceptor substrate
-
-
?
maltose + salicin
glucosyl-alpha-1,6-salicin + isomaltosyl-alpha-1,6-salicin
show the reaction diagram
-
-
-
?
maltose + starch
?
show the reaction diagram
-
starch is the donor substrate, maltose the acceptor substrate
-
-
?
maltose-dextran mixture
4-O-alpha-isomaltosyl-D-glucose + 4-O-alpha-isomaltotriosyl-D-glucose + 4-O-alpha-isomaltotetraosyl-D-glucose + 4-O-alpha-isomaltopentaosyl-D-glucose + 4-O-alpha-isomaltohexaosyl-D-glucose
show the reaction diagram
-
-
-
?
maltotetraitol
dextran
show the reaction diagram
-
-
-
?
maltotetraose
dextran
show the reaction diagram
maltotetraose
maltose + panose
show the reaction diagram
-
-
-
?
maltotriitol
dextran
show the reaction diagram
-
no activity with maltose
-
?
maltotriose
dextran
show the reaction diagram
methyl-alpha-D-glucoside + starch
methyl-alpha-isomaltoside + ?
show the reaction diagram
-
starch is the donor substrate, methyl-alpha-D-glucoside the acceptor substrate
-
-
?
methyl-beta-D-glucoside + starch
methyl-beta-isomaltoside + ?
show the reaction diagram
-
starch is the donor substrate, methyl-beta-D-glucoside the acceptor substrate
-
-
?
nigerose + starch
?
show the reaction diagram
-
starch is the donor substrate, nigerose the acceptor substrate
-
-
?
salicin + starch
?
show the reaction diagram
-
starch is the donor substrate, salicin, i.e. 2-(hydroxymethyl)phenyl-beta-D-glucopyranoside, the acceptor substrate
-
-
?
short-chain amylose
dextran
show the reaction diagram
soluble starch
dextran
show the reaction diagram
-
-
dextran yield 21.4%
?
sophorose + starch
?
show the reaction diagram
-
starch is the donor substrate, sophorose the acceptor substrate
-
-
?
starch + 2-acetamido-2-deoxy-D-glucose
?
show the reaction diagram
-
-
-
-
?
starch + 2-deoxy-D-glucose
?
show the reaction diagram
-
-
-
-
?
starch + 3-O-methyl-D-glucose
?
show the reaction diagram
-
-
-
-
?
starch + 6-deoxy-D-glucose
?
show the reaction diagram
-
-
-
-
?
starch + D-glucose
maltose + isomaltose
show the reaction diagram
-
no transglucosylation from starch to: D-mannose, D-allose, D-galactose, 3-acetamido-3-deoxy-D-glucose, 6-acetamido-6-deoxy-D-glucose, 1,6-anhydro-D-glucose, D-fructose, D-arabinose or D-ribose
-
?
starch + D-xylose
glucosyl-alpha-1,4-xylose + ?
show the reaction diagram
-
-
-
?
starch + salicin
?
show the reaction diagram
-
starch is the donor substrate, salicin the acceptor substrate
-
-
?
starch + salicin
dextran
show the reaction diagram
-
-
-
?
sucrose + starch
?
show the reaction diagram
-
starch is the donor substrate, sucrose the acceptor substrate
-
-
?
xylosucrose + starch
?
show the reaction diagram
-
starch is the donor substrate, xylosucrose the acceptor substrate
-
-
?
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m
[(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
slight activation
Ca2+
-
slight activation
Mg2+
-
slight activation
Mn2+
-
slight activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cd2+
-
partial inhibition at 1 mM
Fe2+
-
strong inhibition
Fe3+
-
0.5 mM, 83% inhibition
KMnO4
maltose
-
strong inhibition of dextran synthesis
additional information
-
not inhibited by Ca2+, Co2+, Fe2+, Mg2+, Mn2+, Ni2+, Zn2+, p-chloromercuribenzoate and EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0017
maltoheptaose
-
-
0.0026
maltohexaose
-
-
0.0033
maltopentaose
-
-
0.0064
maltotetraose
-
-
0.01
maltotriose
-
-
0.00012
short-chain amylose
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
112.5
-
purified enzyme, pH 6.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.2
-
broad optimum
6.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 5.7
-
about half-maximal activity at pH 3.0 and 5.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11 - 35
-
sharp decrease at temperatures higher than 35 C
17 - 52
-
approx. half-maximal activity at 17C and 52C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 5.2
-
30 min stable
488876
4.1 - 5.4
5 - 9
-
stable within this range for 3 h
718616
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable below 30C for 3 h
55
-
inactivation within 30 min
70
-
inactivation within 10 min
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethylene glycol
-
40% v/v in acetate buffer, stable to
n-Butanol
-
H2O-saturated, 50% v/v in acetate buffer, stable to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
extra- and intracellular enzymes
-
native DDase 187.5fold to homogeneity by anion exchange chromatography and gel filtration
-
one-step centrifugation at 4C, 20000 g for 20 min
-
phenyl-Toyopearl, gel filtration
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-CodonPlus-RIL by nickel affinity chromatography, dialysis, and ultrafiltration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)-CodonPlus-RIL
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
enzyme is utilized for bioconversions of glycosides in diverse purposes, overview, and for production of dextran and oligodextran, no product inhibition, enzyme is robust in high level production