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1,5-anhydroglucitol + glucose-1-phosphate
?
-
-
-
-
?
alpha-D -glucose 1-fluoride + D-glucose
cellobiose + fluoride
-
-
-
-
ir
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
? + phosphate
alpha-D-glucose 1-phosphate + 2-deoxy-2-fluoro-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
? + phosphate
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + 5a-carba-alpha-D,L-glucopyranose
4-O-beta-D-glucopyranosyl-5a-carba-beta-D-glucopyranose + phosphate
-
very poor substrate
-
-
?
alpha-D-glucose 1-phosphate + 5a-carba-beta-D,L-glucopyranose
?
-
much better substrate than alpha-anomer, 22% of activity with D-glucose
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-6-fluoro-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
? + phosphate
alpha-D-glucose 1-phosphate + butyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
? + phosphate
-
no substrate for wilalpha-D-type
-
-
r
alpha-D-glucose 1-phosphate + D-altrose
?
alpha-D-glucose 1-phosphate + D-arabinose
?
alpha-D-glucose 1-phosphate + D-arabinose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-fructose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-galactose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-gentobiose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucosamine
4-O-beta-D-glucopyranosyl-D-glucosamine
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
alpha-D-glucose 1-phosphate + D-glucosamine
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucosamine
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-maltose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
4-O-beta-D-glucopyranosyl-D-mannose
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-mannose
?
alpha-D-glucose 1-phosphate + D-mannose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
?
alpha-D-glucose 1-phosphate + D-xylose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + ethyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + L-fucose
?
alpha-D-glucose 1-phosphate + L-galactose
?
alpha-D-glucose 1-phosphate + methyl alpha-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
4-O-beta-D-glucopyranosyl-N-acetyl-D-glucosamine
-
substrate for mutant Y648V only
-
-
?
alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
phosphate + ?
-
-
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
? + phosphate
-
no substrate for wild-type
-
-
r
Avicel + phosphate
? + D-glucose
beta-D-glucose 1-phosphate + alpha-D-glucopyranosyl fluoride
beta-cellobiose + HF
-
beta-D-glucopyranosyl fluoride is not ulilized
-
?
cellobiose + arsenate
D-glucose + alpha-D-glucose 1-arsenate
-
-
alpha-D-glucose 1-arsenate hydrolyses spontaneously to form D-glucose and arsenate
ir
cellobiose + phosphate
alpha-D-glucose 1 phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellopentaose + phosphate
?
63.3% activity compared to cellotetraose
-
-
r
cellotetraose + phosphate
?
100% activity
-
-
r
cellotriose + phosphate
?
89.7% activity compared to cellotetraose
-
-
r
D-glucal + 2-deoxy-2-(hydroxymethyl)-beta-D-xylopyranose
2-deoxy-3-O-(2-deoxy-beta-D-arabino-hexopyranosyl)-2-(hydroxymethyl)-beta-D-xylopyranose + ?
-
-
-
-
r
D-glucal + D-glucose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-glucose + ?
-
-
-
-
r
D-glucal + D-mannose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-mannose + ?
-
-
-
-
r
D-glucal + D-xylose
2-deoxy-beta-D-arabino-hexopyranosyl-(1->4)-D-xylose + ?
-
-
-
-
r
D-glucal + phosphate
2-deoxy-alpha-D-glucose 1-phosphate
-
D-glucal is a slow alternative donor substrate for stereospecific glycosyl transfer to phosphate. Reaction follows a stereochemical course where substrate becomes protonated from below its six-membered ring through stereoselective re side attack at C-2. The proposed catalytic mechanism involves direct protonation of D-glucal by the enzyme-bound phosphate, which then performs nucleophilic attack on the reactive C-1 of donor substrate. When offered D-glucose next to D-glucal and phosphate, the enzyme produces 2-deoxy-beta-D-glucosyl-(1->4)-D-glucose and 2-deoxy-alpha-D-glucose 1-phosphate in a ratio governed by mass action of the two acceptor substrates present. Enzymatic synthesis of 2-deoxy-beta-D-glucosyl-(1->4)-D-glucose is effectively promoted by catalytic concentrations of phosphate, suggesting that catalytic reaction proceeds through a quaternary complex of enzyme, D-glucal, phosphate,and D-glucose
-
-
?
gentiobiose + alpha-D-glucose 1-phosphate
?
-
-
-
-
?
gentiobiose + phosphate
?
-
-
-
r
isomaltose + alpha-D-glucose 1-phosphate
?
-
-
-
-
?
lactose + phosphate
alpha-D-galactose 1-phosphate + D-glucose
-
-
-
r
melibiose + alpha-D-glucose 1-phosphate
?
-
-
-
-
?
phosphoric acid swollen cellulose + phosphate
? + D-glucose
sugarcane bagasse + phosphate
? + D-glucose
additional information
?
-
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
poor substrate
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
much better substrate than 2-deoxy-D-glucose
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + 6-deoxy-D-glucose
? + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-altrose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-altrose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-arabinose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-arabinose
?
-
very poor substrate
-
-
?
alpha-D-glucose 1-phosphate + D-arabinose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
-
poor substrate
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucosamine
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
in synthesis reaction, glucose is best substrate
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
in synthesis reaction, glucose is best substrate
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
cellobiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-mannose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-mannose
?
-
poor substrate
-
-
?
alpha-D-glucose 1-phosphate + D-mannose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-mannose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
no activity with L-isomer
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
poor substrate
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
no activity with L-isomer
-
-
?
alpha-D-glucose 1-phosphate + D-xylose
?
-
higher specific activity than with D-glucose as acceptor
-
-
?
alpha-D-glucose 1-phosphate + L-fucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + L-fucose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + L-galactose
?
-
-
-
-
?
alpha-D-glucose 1-phosphate + L-galactose
?
-
-
-
-
?
Avicel + phosphate
? + D-glucose
-
-
-
-
?
Avicel + phosphate
? + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-cellobiose preferred over alpha-anomer
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
the enzyme shows beta-anomer selective glucosylation activity towards disaccharides
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-cellobiose preferred over alpha-anomer
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
the enzyme shows beta-anomer selective glucosylation activity towards disaccharides
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-D-cellobiose is phosphorylyzed faster than alpha-anomer, ordered bi-bi mechanism
beta-D-glucose was shown to be the 4 times better acceptor compared to alpha-D-glucose in the reverse reaction
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
sequential bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
CBP catalyzes the phosphorolysis of cellobiose into alpha-D-glucose 1-phosphate and D-glucose with inversion of anomeric configuration, computational docking, overview
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
beta-cellobiose preferred over alpha-anomer
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
ternary-complex kinetic mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
important for energy efficient catabolism of cellobiose in cytoplasm
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
Fomes annosus
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
38.6% activity compared to cellotetraose
-
-
r
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
random ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
sequential bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
random ordered bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
sequential bi-bi mechanism
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
O52504
-
-
?
cellobiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
O52504
nonconventional pathway for glucan utilization
-
-
?
phosphoric acid swollen cellulose + phosphate
? + D-glucose
-
-
-
-
?
phosphoric acid swollen cellulose + phosphate
? + D-glucose
-
-
-
-
?
sugarcane bagasse + phosphate
? + D-glucose
-
-
-
-
?
sugarcane bagasse + phosphate
? + D-glucose
-
-
-
-
?
additional information
?
-
beta-glucosylation of alkyls by the enzyme using either cellobiose or alpha-D-glucose 1-phosphate as donors, active with methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, tert-butanol, n-pentanol, n-hexanol, n-heptanol, 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol
-
-
?
additional information
?
-
-
beta-glucosylation of alkyls by the enzyme using either cellobiose or alpha-D-glucose 1-phosphate as donors, active with methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, tert-butanol, n-pentanol, n-hexanol, n-heptanol, 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol
-
-
?
additional information
?
-
beta-glucosylation of alkyls by the enzyme using either cellobiose or alpha-D-glucose 1-phosphate as donors, active with methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, tert-butanol, n-pentanol, n-hexanol, n-heptanol, 1,2-butanediol, 1,3-butanediol, and 1,4-butanediol
-
-
?
additional information
?
-
-
no activity with D-xylose and D-mannose
-
-
?
additional information
?
-
-
no activity detected with glucose-acceptor different from D-glucose
-
-
?
additional information
?
-
only disaccharide accepted as substrate
-
-
?
additional information
?
-
-
only disaccharide accepted as substrate
-
-
?
additional information
?
-
only disaccharide accepted as substrate
-
-
?
additional information
?
-
beyond its cellobiose phosphorylase activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than 2 as a glucose acceptor, releasing phosphate from alpha-D-glucose 1-phosphate
-
-
-
additional information
?
-
-
beyond its cellobiose phosphorylase activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than 2 as a glucose acceptor, releasing phosphate from alpha-D-glucose 1-phosphate
-
-
-
additional information
?
-
O52504
only disaccharide accepted as substrate
-
-
?
additional information
?
-
-
only disaccharide accepted as substrate
-
-
?
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9.5
1,5-anhydro-D-glucitol
-
pH 6.0, 37°C
8 - 9
1,5-anhydroglucitol
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
17.2
2-deoxy-2-fluoro-D-glucose
-
-
24.3 - 250
2-deoxy-D-glucose
7.4
6-deoxy-6-fluoro-D-glucose
-
-
4.1 - 24
6-deoxy-D-glucose
1.46 - 30.7
alpha-D-glucose 1-phosphate
33.7
butyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.06
cellopentaose
at pH 7.5 and 75°C
0.062
cellotetraose
at pH 7.5 and 75°C
0.094
cellotriose
at pH 7.5 and 75°C
10.8
D-gentobiose
-
pH 6.0, 37°C
138 - 155
ethyl beta-D-glucoside
83
gentiobiose
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
71
isomaltose
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
93
melibiose
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
1009
methyl alpha-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
56.6 - 79.8
methyl beta-D-glucoside
17.4
N-acetyl-D-glucosamine
-
mutant enzyme Y648V, at pH 6.0 and 37°C
31.2
phenyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
additional information
additional information
-
simulation of molecular dynamics of the enzyme, the 1S3 pre-transition state conformer is highly stable compared with other conformers, and a conformational change from 4C1 to 1,4B occurs
-
24.3
2-deoxy-D-glucose
-
-
56.9
2-deoxy-D-glucose
-
-
60.2
2-deoxy-D-glucose
-
pH 6.0, 37°C
60.2
2-deoxy-D-glucose
-
wild type enzyme, at pH 6.0 and 37°C
83.1
2-deoxy-D-glucose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
164
2-deoxy-D-glucose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
168
2-deoxy-D-glucose
-
-
250
2-deoxy-D-glucose
-
-
4.1
6-deoxy-D-glucose
-
-
6.1
6-deoxy-D-glucose
-
-
9.2
6-deoxy-D-glucose
-
-
9.8
6-deoxy-D-glucose
-
pH 6.0, 37°C
10.2
6-deoxy-D-glucose
-
-
1.46
alpha-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 37°C
1.57
alpha-D-glucose 1-phosphate
at pH 7.5 and 75°C
2.15
alpha-D-glucose 1-phosphate
-
-
2.15
alpha-D-glucose 1-phosphate
-
pH 6.6, 30°C, native enzyme
3.28
alpha-D-glucose 1-phosphate
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
8
alpha-D-glucose 1-phosphate
-
-
11.3
alpha-D-glucose 1-phosphate
-
mutant enzyme C485A, at pH 6.0 and 37°C
15.7
alpha-D-glucose 1-phosphate
-
mutant enzyme Y648V, at pH 6.0 and 37°C
30.7
alpha-D-glucose 1-phosphate
-
mutant enzyme Y648F, at pH 6.0 and 37°C
0.12
cellobiose
at pH 7.5 and 75°C
0.49
cellobiose
-
at pH 6.0 and 30°C
0.65
cellobiose
-
at pH 6.0 and 30°C
1.68
cellobiose
-
wild type enzyme, at pH 6.0 and 37°C
2.7
cellobiose
pH 6.6, 37°C, recombinant mutant T508I/N667A
2.9
cellobiose
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
2.9
cellobiose
pH 6.6, 37°C, recombinant mutant T508A/N667T
2.9
cellobiose
pH 6.6, 37°C, recombinant wild-type enzyme
3.3
cellobiose
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S
4.3
cellobiose
-
pH 6.6, 30°C, native enzyme
6.9
cellobiose
-
mutant S411G, pH 7.2, 40°C
10.3
cellobiose
-
mutant A423S, pH 7.2, 40°C
10.8
cellobiose
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
11
cellobiose
-
mutant A781 K, pH 7.2, 40°C
14.7
cellobiose
-
mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C
15.9
cellobiose
-
mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C
16.5
cellobiose
-
wild-type, pH 7.2, 40°C
16.6
cellobiose
-
mutant enzyme C485A, at pH 6.0 and 37°C
31.4
cellobiose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
5.7
D-glucosamine
-
-
13.3
D-glucosamine
-
pH 6.0, 37°C
13.3
D-glucosamine
-
wild type enzyme, at pH 6.0 and 37°C
58.1
D-glucosamine
-
mutant enzyme C485A, at pH 6.0 and 37°C
0.69
D-glucose
-
-
1.46
D-glucose
-
pH 6.0, 37°C
2.26
D-glucose
-
pH 6.6, 30°C, native enzyme
3.79
D-glucose
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
14.3
D-glucose
at pH 7.5 and 75°C
17.2
D-glucose
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
28.9
D-glucose
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
11.8
D-mannose
-
mutant enzyme C485A, at pH 6.0 and 37°C
23.5
D-mannose
-
pH 6.0, 37°C
23.5
D-mannose
-
wild type enzyme, at pH 6.0 and 37°C
62.6
D-mannose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
200
D-mannose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
14
D-xylose
-
-
25.5
D-xylose
-
pH 6.0, 37°C
25.7
D-xylose
at pH 7.5 and 75°C
138
ethyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
155
ethyl beta-D-glucoside
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
228
lactose
pH 6.6, 37°C, recombinant wild-type enzyme
230
lactose
pH 6.6, 37°C, recombinant mutant T508I/N667A
254
lactose
pH 6.6, 37°C, recombinant mutant T508A/N667T
330
lactose
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S
56.6
methyl beta-D-glucoside
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
79.8
methyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.15
phosphate
-
-
0.82
phosphate
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
1.2
phosphate
-
pH 6.6, 30°C, native enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.8
1,5-anhydro-D-glucitol
-
pH 6.0, 37°C
3.5
1,5-anhydroglucitol
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
8
2-deoxy-2-fluoro-D-glucose
-
-
7.83 - 119
2-deoxy-D-glucose
44.6
6-deoxy-6-fluoro-D-glucose
-
-
17 - 124
6-deoxy-D-glucose
1.3 - 96.5
alpha-D-glucose 1-phosphate
9
butyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.34
cellopentaose
at pH 7.5 and 75°C
0.49
cellotetraose
at pH 7.5 and 75°C
0.28
cellotriose
at pH 7.5 and 75°C
4
D-gentobiose
-
pH 6.0, 37°C
5.1 - 8.7
ethyl beta-D-glucoside
7.2
gentiobiose
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
1.9
isomaltose
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
2.3
melibiose
-
enzyme assay at 37°C in 50 mM Tris/HCl (pH 7.0)
6
methyl alpha-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
6.8 - 7.9
methyl beta-D-glucoside
6.54
N-acetyl-D-glucosamine
-
mutant enzyme Y648V, at pH 6.0 and 37°C
4
phenyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
7.83
2-deoxy-D-glucose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
11.6
2-deoxy-D-glucose
-
-
18.3
2-deoxy-D-glucose
-
-
21.5
2-deoxy-D-glucose
-
pH 6.0, 37°C
21.5
2-deoxy-D-glucose
-
wild type enzyme, at pH 6.0 and 37°C
119
2-deoxy-D-glucose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
17
6-deoxy-D-glucose
-
-
55.3
6-deoxy-D-glucose
-
-
59.5
6-deoxy-D-glucose
-
-
124
6-deoxy-D-glucose
-
pH 6.0, 37°C
1.3
alpha-D-glucose 1-phosphate
-
mutant enzyme Y648V, at pH 6.0 and 37°C
7.61
alpha-D-glucose 1-phosphate
at pH 7.5 and 75°C
43.2
alpha-D-glucose 1-phosphate
-
pH 6.6, 30°C, native enzyme
43.2
alpha-D-glucose 1-phosphate
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
64.2
alpha-D-glucose 1-phosphate
-
mutant enzyme Y648F, at pH 6.0 and 37°C
92.3
alpha-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 37°C
96.5
alpha-D-glucose 1-phosphate
-
mutant enzyme C485A, at pH 6.0 and 37°C
0.23
cellobiose
at pH 7.5 and 75°C
1.3
cellobiose
-
mutant S411G, pH 7.2, 40°C
1.5
cellobiose
-
mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C
2.01
cellobiose
pH 6.6, 37°C, recombinant mutant T508I/N667A
2.1
cellobiose
-
mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C
2.6
cellobiose
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
2.61
cellobiose
pH 6.6, 37°C, recombinant mutant T508A/N667T
2.9
cellobiose
-
mutant A423S, pH 7.2, 40°C
2.9
cellobiose
-
mutant A781 K, pH 7.2, 40°C
3.28
cellobiose
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S
3.4
cellobiose
-
wild-type, pH 7.2, 40°C
13.2
cellobiose
pH 6.6, 37°C, recombinant wild-type enzyme
29.1
cellobiose
-
pH 6.6, 30°C, native enzyme
29.1
cellobiose
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
73.9
cellobiose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
79.1
cellobiose
-
wild type enzyme, at pH 6.0 and 37°C
88.9
cellobiose
-
mutant enzyme C485A, at pH 6.0 and 37°C
5.2
D-glucosamine
-
-
9.56
D-glucosamine
-
pH 6.0, 37°C
9.56
D-glucosamine
-
wild type enzyme, at pH 6.0 and 37°C
35.4
D-glucosamine
-
mutant enzyme C485A, at pH 6.0 and 37°C
5.3
D-glucose
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
6.3
D-glucose
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
27.5
D-glucose
-
pH 6.6, 30°C, native enzyme
27.5
D-glucose
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
30.3
D-glucose
at pH 7.5 and 75°C
92.3
D-glucose
-
pH 6.0, 37°C
0.626
D-mannose
-
mutant enzyme C485A, at pH 6.0 and 37°C
2.83
D-mannose
-
pH 6.0, 37°C
2.83
D-mannose
-
wild type enzyme, at pH 6.0 and 37°C
5.27
D-mannose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
58.3
D-mannose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
1.37
D-xylose
at pH 7.5 and 75°C
16.5
D-xylose
-
pH 6.0, 37°C
5.1
ethyl beta-D-glucoside
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
8.7
ethyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.109
lactose
pH 6.6, 37°C, recombinant wild-type enzyme
0.35
lactose
pH 6.6, 37°C, recombinant mutant T508I/N667A
0.53
lactose
pH 6.6, 37°C, recombinant mutant T508A/N667T
1.11
lactose
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S
6.8
methyl beta-D-glucoside
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
7.9
methyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
15.4
phosphate
-
pH 6.6, 30°C, native enzyme
15.4
phosphate
-
pH 6.6, 30°C, recombinant His6-tagged enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.29
1,5-anhydro-D-glucitol
-
pH 6.0, 37°C
0.0198 - 1.43
2-deoxy-D-glucose
12.7
6-deoxy-D-glucose
-
pH 6.0, 37°C
0.0829 - 63.2
alpha-D-glucose 1-phosphate
0.266
butyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
5.67
cellopentaose
at pH 7.5 and 75°C
7.91
cellotetraose
at pH 7.5 and 75°C
2.98
cellotriose
at pH 7.5 and 75°C
0.37
D-gentobiose
-
pH 6.0, 37°C
0.61 - 0.72
D-glucosamine
0.033 - 0.063
ethyl beta-D-glucoside
0.006
methyl alpha-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.099 - 0.12
methyl beta-D-glucoside
0.0376
N-acetyl-D-glucosamine
-
mutant enzyme Y648V, at pH 6.0 and 37°C
0.129
phenyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.0198
2-deoxy-D-glucose
-
mutant enzyme C485A, at pH 6.0 and 37°C
0.0477
2-deoxy-D-glucose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
0.357
2-deoxy-D-glucose
-
wild type enzyme, at pH 6.0 and 37°C
0.36
2-deoxy-D-glucose
-
pH 6.0, 37°C
1.43
2-deoxy-D-glucose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
0.0829
alpha-D-glucose 1-phosphate
-
mutant enzyme Y648V, at pH 6.0 and 37°C
2.09
alpha-D-glucose 1-phosphate
-
mutant enzyme Y648F, at pH 6.0 and 37°C
4.85
alpha-D-glucose 1-phosphate
at pH 7.5 and 75°C
8.55
alpha-D-glucose 1-phosphate
-
mutant enzyme C485A, at pH 6.0 and 37°C
63.2
alpha-D-glucose 1-phosphate
-
wild type enzyme, at pH 6.0 and 37°C
0.0681
cellobiose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
0.103
cellobiose
-
mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C
0.134
cellobiose
-
mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C
0.189
cellobiose
-
mutant S411G, pH 7.2, 40°C
0.2
cellobiose
-
wild-type, pH 7.2, 40°C
0.24
cellobiose
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.263
cellobiose
-
mutant A781 K, pH 7.2, 40°C
0.287
cellobiose
-
mutant A423S, pH 7.2, 40°C
0.9
cellobiose
pH 6.6, 37°C, recombinant mutant T508A/N667T
0.99
cellobiose
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S
1.21
cellobiose
pH 6.6, 37°C, recombinant mutant T508I/N667A
1.92
cellobiose
at pH 7.5 and 75°C
2.36
cellobiose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
4.56
cellobiose
pH 6.6, 37°C, recombinant wild-type enzyme
5.35
cellobiose
-
mutant enzyme C485A, at pH 6.0 and 37°C
47.1
cellobiose
-
wild type enzyme, at pH 6.0 and 37°C
0.61
D-glucosamine
-
mutant enzyme C485A, at pH 6.0 and 37°C
0.719
D-glucosamine
-
wild type enzyme, at pH 6.0 and 37°C
0.72
D-glucosamine
-
pH 6.0, 37°C
0.031
D-glucose
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.217
D-glucose
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
2.12
D-glucose
at pH 7.5 and 75°C
63.2
D-glucose
-
pH 6.0, 37°C
0.0263
D-mannose
-
mutant enzyme Y648V, at pH 6.0 and 37°C
0.0532
D-mannose
-
mutant enzyme C485A, at pH 6.0 and 37°C
0.112
D-mannose
-
wild type enzyme, at pH 6.0 and 37°C
0.12
D-mannose
-
pH 6.0, 37°C
0.931
D-mannose
-
mutant enzyme Y648F, at pH 6.0 and 37°C
0.05
D-xylose
at pH 7.5 and 75°C
0.65
D-xylose
-
pH 6.0, 37°C
0.033
ethyl beta-D-glucoside
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
0.063
ethyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.00048
lactose
pH 6.6, 37°C, recombinant wild-type enzyme
0.0015
lactose
pH 6.6, 37°C, recombinant mutant T508I/N667A
0.0021
lactose
pH 6.6, 37°C, recombinant mutant T508A/N667T
0.0034
lactose
pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S
0.099
methyl beta-D-glucoside
-
mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C
0.12
methyl beta-D-glucoside
-
mutant T508I/E649C/N667A, pH 6.6, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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A423S
-
mutation introduced to increase thermostability, mutant displays a half-life of 11.3 min at 70°C
A781K
-
mutation introduced to increase thermostability, mutant displays a half-life of 15.3 min at 70°C
Q130H/K131Y/S411G/A423S/A781K
-
mutations introduced to increase thermostability, mutant displays a half-life of 17.7 min at 70°C
R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K
-
mutations introduced to increase thermostability, mutant displays a half-life of 24.6 min at 70°C
S411G
-
mutation introduced to increase thermostability, mutant displays a half-life of 13.6 min at 70°C
A397V
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows reduced activity with lactose compared to the wild-type enzyme
A397V/T508A/A512T/D557N/N667T/G681S
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows 3fold increased activity with lactose and 6fold decreased activity with cellobiose compared to the wild-type enzyme
A512T
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows similar activity with lactose compared to the wild-type enzyme
D557N
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows similar activity with lactose compared to the wild-type enzyme
E649C
-
mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates
G681S
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows reduced activity with lactose compared to the wild-type enzyme
N156D/N163D/T508I/E649G/N667A
-
mutant with very broad acceptor specificity
T508A/N667T
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows increased activity with lactose compared to the wild-type enzyme
T508I/E649C/N667A
-
triple mutation broadens acceptor specificity
C485A
-
the mutant shows 43% of wild type activity with cellobiose. The mutant shows higher preference for D-glucosamine than the wild type enzyme
Y648F
-
The mutant shows 23% of wild type activity with cellobiose. Apparent kcat/Km values of the mutant for D-mannose and 2-deoxy-D-glucose are 8.2 and 4.0fold higher than those of the wild type enzyme, respectively
Y648V
-
the mutant shows 0.888% of wild type activity with cellobiose and has synthetic activity toward N-acetyl-D-glucosamine
Y47H
-
the mutation is responsible for improved cellobiose consumption in the presence of xylose. The mutation alters the conformation of the enzyme dimer complex to reduce xylose binding to the active site
Y47H/K256/E270D/I384L/N488D/N578S/Y631F
-
the mutant strain shows improved growth and cellobiose consumption in comparison to the strain expressing wild type enzyme
T508I/N667A
random, site-saturation, and site-directed mutagenesis, the mutant enzyme shows 7.5fold increased activity with lactose compared to the wild-type enzyme
T508I/N667A
-
mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates
additional information
-
method development using the enzyme for production of D-glucose 1-phosphate, which is an expensive substrate for synthesis of amylose by alpha-glucan phosphorylase, overview
additional information
random, site-saturation, and site-directed mutagenesis for directed evolution in order to create enzyme variants with significantly increased lactose phosphorylase activity, useful for the production of alpha-D-galactose 1-phosphate, overview. Location of mutations in the 3D structure, overview
additional information
-
random, site-saturation, and site-directed mutagenesis for directed evolution in order to create enzyme variants with significantly increased lactose phosphorylase activity, useful for the production of alpha-D-galactose 1-phosphate, overview. Location of mutations in the 3D structure, overview
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