Information on EC 2.4.1.201 - alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.201
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RECOMMENDED NAME
GeneOntology No.
alpha-1,6-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R = UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
show the reaction diagram
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannosyl-glycoprotein N-acetylglucosaminyltransferases
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N-Glycan biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-glycoprotein 4-beta-N-acetyl-D-glucosaminyltransferase
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor (click here for diagram).
CAS REGISTRY NUMBER
COMMENTARY hide
119699-68-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
duck
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Manually annotated by BRENDA team
turkey
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Manually annotated by BRENDA team
no activity in african green monkey
COS-1 cells
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-
Manually annotated by BRENDA team
no activity in Bos taurus
kidney
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-
Manually annotated by BRENDA team
no activity in Homo sapiens
colon
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Manually annotated by BRENDA team
no activity in Rattus norvegicus
colon and liver
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-
Manually annotated by BRENDA team
no activity in Sus scrofa
colon and stomach
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-
Manually annotated by BRENDA team
medaka fish
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
the enzyme contributes to the surface expression of CD133 through the processing of multiantennary N -linked glycans on CD133
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
show the reaction diagram
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-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-octyl
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-glucosyl-octyl
show the reaction diagram
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synthetic acceptor substrate
identification and large scale preparation of the product
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-(CH2)8-CH3
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-(CH2)8-CH3
show the reaction diagram
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synthetic acceptor substrate
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?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-(CH2)8-COOH
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-(CH2)8-COOH
show the reaction diagram
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synthetic acceptor substrate
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?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-pyridylamine
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-pyridylamine
show the reaction diagram
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fluorescent synthetic substrate
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?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
show the reaction diagram
Q5T4J0
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-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
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0.0125 mM, in the presence of 0.125 M N-acetylglucosamine
ATP
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0.0125 mM, in the presence of 0.125 M N-acetylglucosamine
N-acetylglucosamine
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weak, 0.125 M
UDP
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0.0125 mM, in the presence of 0.125 M N-acetylglucosamine
UDP-hexanolamine
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0.0125 mM, in the presence of 0.125 M N-acetylglucosamine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09
N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R
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0.6
UDP-N-acetyl-D-glucosamine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000045
recombinant enzyme in COS-1 cells
0.032
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purified enzyme
additional information
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assay method development
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.4
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about half-maximal activity at pH 5.0 and about 80% of maximal activity at pH 8.4
additional information
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broad range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
tissue distribution
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
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SDS-PAGE
additional information
type II transmembrane protein, primary structure, amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithiothreitol is required for stabilization of the purified enzyme
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enzyme becomes more stable after elution from a Ni2+-chelating column during purification
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+ -chelating Sepharose FF column
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning from cDNA library, DNA sequence determination, functional expression in COS-1 cells
expressed in sf21 cells
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gene MGAT6, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat6 HEK293 cells produce increased oligosaccharide levels also observed in HeLa cells, predicted to be the 2,2,4,6 tetra-, 4,2,2,4 tetra- and 4,2,2,4,6 penta-N-glycans, while native 4,2,2,6 tetra are reduced, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
GlcNAc and tet-induced Mgat6 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions. The Mgat5, EC 2.4.1.155, product is the preferred acceptor for Mgat6, which adds a GlcNAcbeta1,4 branch that is not present in mammals, Mgat6 enhances functionality of N-glycan branching in mammalian cells