Information on EC 2.4.1.206 - lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.206
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RECOMMENDED NAME
GeneOntology No.
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3-beta-N-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
83682-80-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
gene B3gnt5
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-
Manually annotated by BRENDA team
no activity in Spodoptera frugiperda
Sf9 cells
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1-4)-beta-D-glucosyl-(1-1)-ceramide
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-phenyl-C14H29
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-phenyl-C14H29
show the reaction diagram
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synthetic substrate, 75% specific activity compared to lactosylceramide
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?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
?
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
78% activity compared to Mn2+
Cu2+
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4% activity compared to Mn2+
Hg2+
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4% activity compared to Mn2+
Mg2+
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40% activity compared to Mn2+
Zn2+
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11% activity compared to Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
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down-regulation of enzyme expression
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta3Gn-T8
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beta3Gn-T2 is activated by beta3Gn-T8
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CHAPS
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41% stimulation compared to Triton CF-54
deoxycholate
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8% stimulation compared to Triton CF-54
octyl-beta-D-glucopyranoside
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46% stimulation compared to Triton CF-54
retinoic acid
sodium taurocholate
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25% stimulation compared to Triton CF-54
taurodeoxycholate
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11% stimulation compared to Triton CF-54
Triton CF-54
Triton X-100
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89% stimulation compared to Triton CF-54
Tween 20
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38% stimulation compared to Triton CF-54
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
D-galactosyl-1,4-beta-D-glucosylceramide
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cerebellum microsomes
0.035
D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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cerebellum microsomes
0.42
Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
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betaGn3-T2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000009
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granule cells, 14 days old
0.0000001
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cerebellum homogenate of 3 days old organisms
0.00000015
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granule cells, 3 days old
0.00000034
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colonic mucosa
0.00000042
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cerebellum homogenate of 14 days old organisms
0.00000065
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embryo
0.0000046
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colonic adenocarcinoma tissue sample
0.00121
0.00123
0.00174
0.0021
0.00254
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
7.3
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assay at
7.6
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.4
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cacodylate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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not epithelial cells
Manually annotated by BRENDA team
the enzyme is expressedin two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens
Manually annotated by BRENDA team
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very low content
Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
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Daoy and D341 Med, cultured both in vitro and as xenografts in nude mice
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FLAG-tagged fusion protein from Sf21 insect cells
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; partial sequence, not a full length clone, DNA sequence determination
DNA and amino acid sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
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DNA sequence determination and analysis; expression in Nawalma cells, membrane-bound; soluble expression as FLAG-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
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DNA sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
expressed in COS-7 cells
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expression in Escherichia coli, overexpression of beta3GnT5 in MNK-45 cells and AGS cells, transfection with lipofectamine
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gene B3gnt5, chromosome 16 B1
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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adhesion of Helicobacter pylori to epithelial cells is mediated by bacterial adhesins that bind carbohydrate ligands expressed by the epithelial gastric cells, bea3GnT5 transferase induction is specific to infection with highly pathogenic strains