Information on EC 2.4.1.282 - 3-O-alpha-D-glucosyl-L-rhamnose phosphorylase

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The expected taxonomic range for this enzyme is: Lachnoclostridium phytofermentans

EC NUMBER
COMMENTARY hide
2.4.1.282
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RECOMMENDED NAME
GeneOntology No.
3-O-alpha-D-glucosyl-L-rhamnose phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate = L-rhamnopyranose + beta-D-glucose 1-phosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
3-O-alpha-D-glucopyranosyl-L-rhamnoopyranose:phosphate beta-D-glucosyltransferase
The enzyme does not phosphorylate alpha,alpha-trehalose, kojibiose, nigerose, or maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
L-rhamnose + beta-D-glucose 1-phosphate
show the reaction diagram
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
L-rhamnose + beta-D-glucose 1-phosphate
3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.6
beta-D-glucose 1-phosphate
pH 6.5, 30C, reverse phosphorolysis reaction
2.7
L-rhamnose
pH 6.5, 30C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5
beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
A9KM56
pH 6.5, 30C, reverse phosphorolysis reaction
1.6
L-rhamnose
Lachnoclostridium phytofermentans
A9KM56
pH 6.5, 30C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
87990
calculated from amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli