Information on EC 2.4.1.284 - 2-deoxystreptamine glucosyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces kanamyceticus

EC NUMBER
COMMENTARY hide
2.4.1.284
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RECOMMENDED NAME
GeneOntology No.
2-deoxystreptamine glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 2-deoxystreptamine = UDP + 2'-deamino-2'-hydroxyparomamine
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
kanamycin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:2-deoxystreptamine 6-alpha-D-glucosyltransferase
Involved in the biosynthesis of kanamycin B and kanamycin C. Also catalyses EC 2.4.1.283, 2-deoxystreptamine N-acetyl-D-glucosaminyltransferase, but activity is only one fifth of that with UDP-alpha-D-glucose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-D-glucose + 2-deoxystreptamine
?
show the reaction diagram
GDP-D-mannose + 2-deoxystreptamine
?
show the reaction diagram
UDP-D-galactose + 2-deoxystreptamine
?
show the reaction diagram
UDP-D-glucose + 2-deoxystreptamine
UDP + 2'-deamino-2'-hydroxyparomamine
show the reaction diagram
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best substrate
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?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine
UDP + 2'-N-acetylparomamine
show the reaction diagram
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reaction of 2.4.1.283, one fifth of the catalytic efficiency with UDP-D-glucose
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?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28 - 0.32
2-deoxystreptamine
0.44
UDP-D-glucose
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in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl22, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30C
2.54
UDP-N-acetyl-alpha-D-glucosamine
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in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
UDP-D-glucose
Streptomyces kanamyceticus
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in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30C
0.33
UDP-N-acetyl-alpha-D-glucosamine
Streptomyces kanamyceticus
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in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.66
UDP-D-glucose
Streptomyces kanamyceticus
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in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30C
889
0.13
UDP-N-acetyl-alpha-D-glucosamine
Streptomyces kanamyceticus
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in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30C
1424
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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KanF cannot be functionally expressed in Escherichia coli
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