Information on EC 2.4.1.295 - anthocyanin 3-O-sambubioside 5-O-glucosyltransferase

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The expected taxonomic range for this enzyme is: Matthiola incana

EC NUMBER
COMMENTARY hide
2.4.1.295
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RECOMMENDED NAME
GeneOntology No.
anthocyanin 3-O-sambubioside 5-O-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-sambubioside = UDP + an anthocyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
anthocyanidin sambubioside biosynthesis
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Anthocyanin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:anthocyanidin-3-O-beta-D-sambubioside 5-O-glucosyltransferase
Isolated from the plant Matthiola incana (stock). No activity with anthocyanidin 3-O-glucosides.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
different lines, overview; R.Br.
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-alpha-D-glucose + cyanidin 3-O-beta-D-sambubioside
dTDP + cyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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?
dTDP-alpha-D-glucose + pelargonidin 3-O-beta-D-sambubioside
dTDP + pelargonidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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?
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-sambubioside
UDP + an anthocyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-(4-coumaroyl))-beta-D-sambubioside
UDP + cyanidin 5-O-beta-D-glucosyl-3-O-(6-O-(4-coumaroyl))-beta-D-sambubioside
show the reaction diagram
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?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-sambubioside
UDP + cyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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?
UDP-alpha-D-glucose + pelargonidin 3-O-(4-coumaroyl)-beta-D-sambubioside
UDP + pelargonidin 5-O-beta-D-glucoside 3-O-beta-(4-coumaroyl)-D-sambubioside
show the reaction diagram
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?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-sambubioside
UDP + pelargonidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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?
additional information
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substrate specificity, overview. The enzyme catalyzes the transfer of the glucosyl moiety of uridine 5'-diphosphoglucose to the 5-hydroxyl group of pelargonidin and cyanidin 3-glycosides and acylated derivatives. The best substrate for 5-glucosylation is the 3-xylosylglucoside acylated with 4-coumarate, followed by the 3-xylosylglucoside and by the acylated (4-coumarate) 3-glucoside. The 3-glucosides themselves are a very poor substrates. No activity with UDP-xylose, -galactose, -glucuronic acid and -mannose, ADP-glucose, GDP-glucose, and CDP-glucose
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-sambubioside
UDP + an anthocyanidin 5-O-beta-D-glucoside 3-O-beta-D-sambubioside
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no effect by Ca2+, Mg2+, and KCN
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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32% inhibition at 1 mM
Cu2+
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complete inhibition at 1 mM
diethyl dicarbonate
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20% inhibition at 2 mM
EDTA
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32% inhibition at 2 mM
Fe2+
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41% inhibition at 1 mM
Mn2+
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10% inhibition at 1 mM
Zn2+
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50-60% inhibition at 1 mM
additional information
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no inhibition by diethyl dithiocarbamate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
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activity range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
genotyping
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