Information on EC 2.4.1.297 - anthocyanidin 3-O-glucoside 2''-O-glucosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.297
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RECOMMENDED NAME
GeneOntology No.
anthocyanidin 3-O-glucoside 2''-O-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-glucoside = UDP + an anthocyanidin 3-O-sophoroside
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Anthocyanin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucose:anthocyanidin-3-O-glucoside 2''-O-glucosyltransferase
Isolated from Ipomoea nil (Japanese morning glory).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Japanese morning glory
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-galactoside
UDP + cyanidin 2''-O-alpha-D-glucoside 3-O-beta-D-galactoside
show the reaction diagram
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9.1% of the activity with cyanidin 3-O-beta-D-glucoside
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?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-sophoroside
show the reaction diagram
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?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
UDP + delphinidin 3-O-sophoroside
show the reaction diagram
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64.8% of the activity with cyanidin 3-O-beta-D-glucoside
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?
UDP-alpha-D-glucose + kaempferol 3-O-beta-D-glucoside
UDP + kaempferol 3-O-sophoroside
show the reaction diagram
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41.6% of the activity with cyanidin 3-O-beta-D-glucoside
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?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
UDP + pelargonidin 3-O-sophoroside
show the reaction diagram
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29.3% of the activity with cyanidin 3-O-beta-D-glucoside
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?
UDP-alpha-D-glucose + quercetin 3-O-beta-D-glucoside
UDP + quercetin 3-O-sophoroside
show the reaction diagram
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33.8% of the activity with cyanidin 3-O-beta-D-glucoside
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?
additional information
?
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enzyme catalyzes only the 2''-O-glucosylation of flavonoid 3-O-glucosideto produce flavonoid 3-O-sophoroside. Both anthocyanidin 3-O-glucosides and flavonol 3-O-glucosides are favorable acceptor substrates of the enzyme, whereas no activity is shown toward C-glucosylflavones such as isovitexin
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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1 mM, 84% residual activity
4-chloromercuribenzoate
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1 mM, 4% residual activity
Co2+
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1 mM, 31% residual activity
Cu2+
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1 mM, 10% residual activity
N-ethylmaleimide
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1 mM, 16.4% residual activity
Zn2+
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1 mM, 3.5% residual activity
additional information
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not inhibitory: EDTA at 1 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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1 mM, 125% of initial activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
82000
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gel fitlration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 82000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE