Information on EC 2.4.1.321 - cellobionic acid phosphorylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.321
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RECOMMENDED NAME
GeneOntology No.
cellobionic acid phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate = alpha-D-glucose 1-phosphate + D-gluconate
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
4-O-beta-D-glucopyranosyl-D-gluconate:phosphate alpha-D-glucosyltransferase
The enzyme occurs in cellulolytic bacteria and fungi. It catalyses the reversible phosphorolysis of cellobionic acid. In the synthetic direction it produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
show the reaction diagram
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
show the reaction diagram
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
show the reaction diagram
alpha-D-glucose 1 phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + D-glucuronate
4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1 phosphate + D-gluconate
show the reaction diagram
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
alpha-D-glucose 1-phosphate + D-gluconate
show the reaction diagram
alpha-D-glucose 1 phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
show the reaction diagram
alpha-D-glucose 1-phosphate + D-gluconate
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-O-beta-D-glucopyranosyl-D-gluconate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
4-O-beta-D-glucopyranosyl-D-gluconate
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pH 7.0, 30C
0.21
alpha-D-glucose 1-phosphate
1.7 - 41
D-gluconate
2.4 - 13.4
D-glucuronate
0.1
phosphate
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pH 7.0, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
306
4-O-beta-D-glucopyranosyl-D-gluconate
Neurospora crassa
-
pH 7.0, 30C
127
alpha-D-glucose 1-phosphate
24 - 173
D-gluconate
34.6 - 43
D-glucuronate
306
phosphate
Neurospora crassa
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pH 7.0, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
605
alpha-D-glucose 1-phosphate
Xanthomonas campestris
Q8P3J4
pH 6.5, 30C
107
0.58 - 87
D-gluconate
698
2.58 - 18
D-glucuronate
339
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.3
4-O-beta-D-glucopyranosyl-D-gluconate
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pH 7.0, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
substrate 4-O-beta-D-glucopyranosyl-D-gluconate, mutant Q190A, pH 6.5, 30C
62
substrate 4-O-beta-D-glucopyranosyl-D-gluconate, wild-type, pH 6.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
synthetic activity; synthetic reaction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
147000
gel filtration
171000
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gel filtration
179000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structures of ligand-free and complex forms with cellobionic acid, gluconic acid, and a synthetic disaccharide product from glucuronic acid, up to 1.6 A resolution. The active site is located near the dimer interface. At subsite +1, the carboxylate group of gluconic acid and cellobionic acid is recognized by residues Arg609 and Lys613. Additionally, one residue from the neighboring protomer (Gln190) is involved. These residues are critical for the binding and catalysis of gluconic acid and glucuronic acid
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
4C, 24 h, stable
725022
5.5 - 9
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; 4C, 24 h, stable
725022
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli; expression in Escherichia coli as a His-tagged fusion protein
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expression in Escherichia coli as a His-tagged fusion protein
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Q190A
about 10% of wild-type activity
R609A
complete loss of activity
R613A
complete loss of activity
Q190A
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about 10% of wild-type activity
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R609A
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complete loss of activity
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R613A
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complete loss of activity
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis