Information on EC 2.4.1.332 - 1,2-alpha-glucosylglycerol phosphorylase

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The expected taxonomic range for this enzyme is: Bacillus selenitireducens

EC NUMBER
COMMENTARY hide
2.4.1.332
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RECOMMENDED NAME
GeneOntology No.
1,2-alpha-glucosylglycerol phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-O-alpha-D-glucopyranosyl-glycerol + phosphate = beta-D-glucose 1-phosphate + glycerol
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
2-O-alpha-D-glucopyranosyl-sn-glycerol:phosphate beta-D-glucosyltransferase
The enzyme has been isolated from the bacterium Bacillus selenitireducens. In the absence of glycerol the enzyme produces alpha-D-glucopyranose and phosphate from beta-D-glucopyranose 1-phosphate. In this reaction the glucosyl residue is transferred to a water molecule with an inversion of the anomeric conformation.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
show the reaction diagram
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
beta-D-glucose 1-phosphate + sn-glycerol
show the reaction diagram
beta-D-glucose 1-phosphate + sn-glycerol
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
2-O-alpha-D-glucopyranosyl-sn-glycerol
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pH 8.0, 30C
0.45
beta-D-glucose 1-phosphate
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pH 7.5, 30C
0.57
phosphate
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pH 8.0, 30C
5.7
sn-glycerol
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pH 7.5, 30C
additional information
beta-D-glucose 1-phosphate
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the Km for beta-D-glucose 1-phosphate in the hydrolytic reaction is 0.041 and 0.16 mM at pH 6.0 and pH 7.5 respectively
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
95
2-O-alpha-D-glucopyranosyl-sn-glycerol
Bacillus selenitireducens
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pH 8.0, 30C
180
beta-D-glucose 1-phosphate
Bacillus selenitireducens
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pH 7.5, 30C
163
sn-glycerol
Bacillus selenitireducens
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pH 7.5, 30C
additional information
beta-D-glucose 1-phosphate
Bacillus selenitireducens
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kcat for beta-D-glucose 1-phosphate in the hydrolytic reaction is 2.8 and 1.7 s(-1) at pH 6.0 and pH 7.5 respectively
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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synthesis of 2-O-alpha-D-glucopyranosyl-sn-glycerol
8
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phosphorolytic activity
additional information
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hydrolysis of beta-D-glucose 1-phosphate has an optimum of pH 6.0
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)
Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
86260
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calculated from amino acid sequence
174000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
obtained at 20C using the hanging drop vapor diffusion method by mixing 1 microL of protein solution containing 4.35.7 mg/mL protein with an equal volume of reservoir solution containing 28% polyethylene glycol 400, 0.1 M HEPES-NaOH (pH 7.5), and 0.2 M CaCl2
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10.5
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4C, 24 h stable
728636
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E475A
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inactive mutant
K587A
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inactive mutant
W381F
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inactive mutant
Y327F
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inactive mutant
E475A
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inactive mutant
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E475Q
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inactive mutant; inactive mutant
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K587A
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inactive mutant
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W381F
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inactive mutant
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Y327F
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inactive mutant
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