Information on EC 2.4.1.334 - 1,3-alpha-oligoglucan phosphorylase

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The expected taxonomic range for this enzyme is: Lachnoclostridium phytofermentans

EC NUMBER
COMMENTARY hide
2.4.1.334
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RECOMMENDED NAME
GeneOntology No.
1,3-alpha-oligoglucan phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
[(1->3)-alpha-D-glucosyl]n + phosphate = [(1->3)-alpha-D-glucosyl]n-1 + beta-D-glucose 1-phosphate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
1,3-alpha-D-glucan:phosphate beta-D-glucosyltransferase
The enzyme, isolated from the bacterium Clostridium phytofermentans, catalyses a reversible reaction. Substrates for the phosphorolytic reaction are alpha-1,3-linked oligoglucans with a polymerisation degree of 3 or more. Nigerose (i.e. 3-O-alpha-D-glucopyranosyl-D-glucopyranose) is not phosphorylyzed but can serve as substrate in the reverse direction (cf. EC 2.4.1.279, nigerose phosphorylase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranosyl 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
kojibiose + beta-D-glucopyranose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
maltose + beta-D-glucopyranose 1-phosphate
? + phosphate
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
3-O-alpha-D-glucopyranosyl-D-glucopyranose + beta-D-glucopyranose 1-phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.9
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
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1 - 3.2
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
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3.1
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
8.3
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
50
kojibiose
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
3.8
maltose
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
2.6 - 11
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.5
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, phosphorolysis reaction, release of beta-D-glucopyranose 1-phosphate
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11 - 17
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
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8.7
3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
5.1
beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
3.5
kojibiose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
2.7
maltose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
3-O-alpha-D-glucopyranosyl-3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
194826
2.8
3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
15208
1.6
beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, acceptor 3-O-alpha-D-glucopyranosyl-D-glucopyranose, i.e. nigerose
194820
0.069
kojibiose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
1470
0.5
maltose
Lachnoclostridium phytofermentans
A9KSL6
pH 7.0, 30C, reverse phosphorolysis reaction, donor beta-D-glucopyranose 1-phosphate
86
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
phosphorolytic activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21