Information on EC 2.4.1.44 - lipopolysaccharide 3-alpha-galactosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.44
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RECOMMENDED NAME
GeneOntology No.
lipopolysaccharide 3-alpha-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + lipopolysaccharide = UDP + 3-alpha-D-galactosyl-[lipopolysaccharide glucose]
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lipid A-core biosynthesis
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lipid A biosynthesis
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Lipopolysaccharide biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:lipopolysaccharide 3-alpha-D-galactosyltransferase
Transfers alpha-D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide [cf. EC 2.4.1.56 (lipopolysaccharide N-acetylglucosaminyltransferase), EC 2.4.1.58 (lipopolysaccharide glucosyltransferase I) and EC 2.4.1.73 (lipopolysaccharide glucosyltransferase II)].
CAS REGISTRY NUMBER
COMMENTARY hide
9073-98-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
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no activity in absence of divalent cations, 20% of the activation with MgCl2
MgCl2
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no activity in absence of divalent cations, highest activity with MgCl2
MnCl2
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no activity in absence of divalent cations, 20% of the activation with MgCl2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Nonsubstrate lipopolysaccharide
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competitive, Salmonella typhimurium SL1032, TV119, LT2 lipopolysaccharides; effect on several strains, overview
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Phospholipid
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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several bacterial strains, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
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4°C, several days, inactivation
489211
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
one cycle of freezing and thawing causes marked loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, pH 6.8, 0.02-0.04 mg/ml protein, stable for at least 1 month
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