Information on EC 2.4.1.74 - glycosaminoglycan galactosyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.74
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RECOMMENDED NAME
GeneOntology No.
glycosaminoglycan galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + glycosaminoglycan = UDP + D-galactosylglycosaminoglycan
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:glycosaminoglycan D-galactosyltransferase
Involved in the biosynthesis of galactose-containing glycosaminoglycan of the ameboid protozoan Dictyostelium discoideum.
CAS REGISTRY NUMBER
COMMENTARY hide
51004-28-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
male albino Sprague-Dawley
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + glycosaminoglycan
UDP + D-galactosylglycosaminoglycan
show the reaction diagram
-
-
-
-
?
UDPgalactose + glycosaminoglycan
UDP + D-galactosylglycosaminoglycan
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPgalactose + glycosaminoglycan
UDP + D-galactosylglycosaminoglycan
show the reaction diagram
-
involved in the biosynthesis of galactose-containing glycosaminoglycan of Dictostelium discoideum
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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stimulates at low UDPgalactose concentration, inhibits at high UDPgalactose concentration
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
14 mM, 2.5fold stimulation
Mn2+
-
maximal activation by 5 mM MnCl2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CuCl2
-
5-10 mM, more than 90% inhibition
EDTA
-
inhibits probably due to complex binding to Mn2+
Lead acetate
-
5-10 mM, more than 90% inhibition
p-chloromercuribenzoate
-
5-10 mM, more than 90% inhibition
Triton X-100
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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stimulation
ATP
-
inhibits at high UDPgalactose concentration, stimulates at low UDPgalactose concentration
Polyethyleneglycol
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enhances activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
UDP-galactose
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8
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pH 6.4: about 50% of maximal activity, pH 8.0: about 90% of maximal activity at pH 8.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 21
-
reaction proceeds faster at 0°C than at 21°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
activity appears shortly before actual synthesis of galactose-containing mucopolysaccharides, it reaches a peak of activity shortly before the end of fruiting body construction and then disappears
Manually annotated by BRENDA team
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specifically localized in. Activity sharply changes in response to differentiation and dedifferentiation
Manually annotated by BRENDA team
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migrating, no activity in vegetative cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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enzyme is specifically localized in the smaller vesicles with lighter density than the prespore-specific vacuoles identifiable electron microscopically
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
200000
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non-denaturing PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
rapid loss of activity is prevented by the presence of albumin or Triton-X
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE