Information on EC 2.4.2.50 - cyanidin 3-O-galactoside 2''-O-xylosyltransferase

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The expected taxonomic range for this enzyme is: Daucus carota

EC NUMBER
COMMENTARY hide
2.4.2.50
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RECOMMENDED NAME
GeneOntology No.
cyanidin 3-O-galactoside 2''-O-xylosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-galactoside = UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-galactoside)
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acylated cyanidin galactoside biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-xylose:cyanidin-3-O-beta-D-galactoside 2''-O-xylosyltransferase
Isolated from the plant Daucus carota (Afghan cultivar carrot).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Afghan cultivar; Afghan cultivar carrot
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-galactoside
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-galactoside)
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-galactoside
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-galactoside)
show the reaction diagram
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-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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divalent cations have no influence on the enzyme activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
cyanidin 3-O-beta-D-galactoside
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pH 7.5, 32°C, crude enzyme
0.2
UDP-alpha-D-xylose
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pH 7.5, 32°C, crude enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially, by ammonium sulfate fractionation and ultracentrifugation, the enzyme is then separated from the UDP-galactose:cyanidin galactosyltransferase, CGT, by anion exchange and affinity chromatography. CGXT activity is lost after another step of gel filtration
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