Information on EC 2.4.2.56 - kaempferol 3-O-xylosyltransferase

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The expected taxonomic range for this enzyme is: Euonymus alatus

EC NUMBER
COMMENTARY hide
2.4.2.56
-
RECOMMENDED NAME
GeneOntology No.
kaempferol 3-O-xylosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-xylose + kaempferol = UDP + kaempferol 3-O-beta-D-xyloside
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:kaempferol 3-O-beta-D-xylosyltransferase
The enzyme from the plant Euonymus alatus also catalyses the 3-O-D-xylosylation of other flavonols (e.g. quercetin, isorhamnetin, rhamnetin, myricetin, fisetin) with lower activity.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + 3,3',4',7-tetrahydroxy-5'-methoxyflavone
UDP + 3,3',4',7-tetrahydroxy-5'-methoxyflavone 3-O-beta-D-xyloside
show the reaction diagram
UDP-alpha-D-xylose + 3,4',7-trihydroxyflavone
UDP + 3,4',7-trihydroxyflavone 3-O-beta-D-xyloside
show the reaction diagram
UDP-alpha-D-xylose + 3,5,7-trihydroxy-4'-methoxyflavone
UDP + 3,5,7-trihydroxy-4'-methoxyflavone 3-O-beta-D-xyloside
show the reaction diagram
UDP-alpha-D-xylose + 3-hydroxy-4',5,7-trimethoxyflavone
UDP + 3-hydroxy-4',5,7-trimethoxyflavone 3-O-beta-D-xyloside
show the reaction diagram
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15% of the activity compared to kaempferol
-
-
?
UDP-alpha-D-xylose + 4',5,7-trihydroxyflavan-3-ol
UDP + 3-O-beta-D-xylopyranosyl-4',5,7-trihydroxyflavan-3-ol
show the reaction diagram
-
25% of the activity compared to kaempferol
-
-
?
UDP-alpha-D-xylose + fisetin
UDP + fisetin 3-O-beta-D-xyloside
show the reaction diagram
-
62% of the activity compared to kaempferol
-
-
?
UDP-alpha-D-xylose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-xyloside
show the reaction diagram
-
85% of the activity compared to kaempferol
-
-
?
UDP-alpha-D-xylose + kaempferol
UDP + kaempferol 3-O-beta-D-xyloside
show the reaction diagram
UDP-alpha-D-xylose + myricetin
UDP + myricetin 3-O-beta-D-xyloside
show the reaction diagram
-
48% of the activity compared to kaempferol
-
-
?
UDP-alpha-D-xylose + quercetin
UDP + quercetin 3-O-beta-D-xyloside
show the reaction diagram
-
76% of the activity compared to kaempferol
-
-
?
UDP-alpha-D-xylose + rhamnetin
UDP + rhamnetin 3-O-beta-D-xyloside
show the reaction diagram
-
67% of the activity compared to kaempferol
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-xylose + kaempferol
UDP + kaempferol 3-O-beta-D-xyloside
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
10 mM, 75% inhibition
Co2+
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1 mM, 96% inhibition
Cu2+
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1 mM, 99% inhibition
iodoacetamide
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1 mM, 44% inhibition
Mg2+
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20 mM, 77% inhibition
Mn2+
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10 mM, 98% inhibition
Phenylmercuriacetate
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1 mM, 98% inhibition
Zn2+
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1 mM, 99% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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strong stimulation even in the presence of Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00083
kaempferol
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pH 7.5, 30C
0.00119
quercetin
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pH 7.5, 30C
0.025
UDP-alpha-D-xylose
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pH 7.5, 30C, cosubstrate: kaempferol
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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Tris-HCl buffer
7.5
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imidazole-HCl buffer
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
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gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 20 mM Tris-HCl buffer, pH 7.5, 14 mM 2-mercaptoethanol and 10% glycerol, 11% loss of activity after 2 days, 55% loss after 8 days, 84% loss after 40 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE