Information on EC 2.4.99.11 - lactosylceramide alpha-2,6-N-sialyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.99.11
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RECOMMENDED NAME
GeneOntology No.
lactosylceramide alpha-2,6-N-sialyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide alpha-(2->6)-N-acetylneuraminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
55071-95-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
20 days old
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cichlid fish
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Manually annotated by BRENDA team
Sprague-Dawley
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
CMP-N-acetylneuraminate + Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)Glcbeta(1<->1)ceramide
CMP + NeuAcalpha(2->6)Galbeta(1->4)GlcNAcbeta(1->3)Galbeta(1->4)Glcbeta(1<->1)ceramide
show the reaction diagram
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Endogenous acidic peptide
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heat-stable, amino acid composition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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the enzyme is induced by phorbol 12-myristate 13-acetate via a PKC/ERK/CREB-dependent pathway in K562 human leukemia cells, the induction is inhibited by Gö6976, a specific inhibitor of PKC, and U0126, an inhibitor of the extracellular signal-regulated kinase, but not by wortmannin, overview
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15
CMP-N-acetylneuraminate
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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a leukemia cell line, the enzyme is expressed during the induction of megakaryocytic differentiation
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homogenized, centrifuged, resuspended in 140 mM-cacodylate/HCl buffer pH 6.8
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homogenized, centrifuged, resuspended in 25 mM-cacodylate buffer pH 6.5 containing 0.15% Triton X-100, 75 mM NaCl and 10 mM MnCl2
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression analysis
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