Information on EC 2.4.99.5 - galactosyldiacylglycerol alpha-2,3-sialyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.99.5
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RECOMMENDED NAME
GeneOntology No.
galactosyldiacylglycerol alpha-2,3-sialyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CMP-N-acetyl-beta-neuraminate + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol = CMP + 1,2-diacyl-3-[3-(N-acetyl-alpha-D-neuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerolipid metabolism
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SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate:1,2-diacyl-3-beta-D-galactosyl-sn-glycerol N-acetylneuraminyltransferase
The beta-D-galactosyl residue of the oligosaccharide of glycoproteins may also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
80237-98-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Swiss-Webster albino
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol
CMP + 1,2-diacyl-3-[3-(alpha-D-N-acetylneuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
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i.e. galactosyldiacylglycerol, transfers N-acetylneuraminic acid to position C-3 of the galactosyl residue
i.e. sialosylgalactosyldiacylglycerol
?
CMP-N-acetylneuraminate + Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc
CMP + ?
show the reaction diagram
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i.e. lacto-N-tetraose
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-
?
CMP-N-acetylneuraminate + Galbeta1-3GlcNAcR
CMP + NeuAcalpha2-3Galbeta1-3GlcNAcR
show the reaction diagram
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i.e. rat asialo alpha1 acid glycoprotein
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-
?
CMP-N-acetylneuraminate + Galbeta1-4GlcNAc
CMP + NeuAcalpha2-3Galbeta1-4GlcNAc
show the reaction diagram
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i.e. N-acetyllactosamine
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?
CMP-N-acetylneuraminate + Galbeta1-4GlcNAcR
CMP + NeuAcalpha2-3Galbeta1-4GlcNAcR
show the reaction diagram
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i.e. human asialo alpha1 acid glycoprotein
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-
?
CMP-N-acetylneuraminic acid + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol
CMP + 1,2-diacyl-3-[3-(alpha-D-N-acetyl-neuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
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-
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-
?
CMP-N-acetylneuraminic acid + NeuAcalpha-(2-3)-Galbeta-(1-4)-GlcNAcbeta-1-octyl
?
show the reaction diagram
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activity of the recombinant enzyme with sialylated beta-D-Gal-(1-3)-alpha-D-GalNAc-octyl containing oligosaccharides, synthesized in analogy to oligosaccharides found on mucin type complex O-glycans, overview
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol
CMP + 1,2-diacyl-3-[3-(alpha-D-N-acetylneuraminyl)-beta-D-galactosyl]-sn-glycerol
show the reaction diagram
-
i.e. galactosyldiacylglycerol, transfers N-acetylneuraminic acid to position C-3 of the galactosyl residue
i.e. sialosylgalactosyldiacylglycerol
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.057 - 0.78
CMP-acetylneuraminate
0.13
galactosyldiacylglycerol
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0.11
Galbeta1-3GlcNAcbeta1-1Galbeta1-4Glc
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0.18
Galbeta1-3GlcNAcR
2.43
Galbeta1-4GlcNAc
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0.26
Galbeta1-4GlcNAcR
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-
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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use for chemoenzymatic synthesis of 13C-labeled sialyloligosaccharide