Information on EC 2.5.1.123 - flaviolin linalyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces cinnamonensis

EC NUMBER
COMMENTARY hide
2.5.1.123
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RECOMMENDED NAME
GeneOntology No.
flaviolin linalyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate + flaviolin = 3-linalylflaviolin + diphosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate:flaviolin 3-linalyltransferase
Does not require Mg2+ or any other metal ions. Isolated from the bacterium Streptomyces cinnamonensis. In vitro the enzyme also forms traces of 3-geranylflaviolin.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + 1,3-dihydroxynaphthalene
3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol
show the reaction diagram
geranyl diphosphate + 4-hydroxybenzoate
4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid
show the reaction diagram
geranyl diphosphate + flaviolin
3-linalylflaviolin + diphosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + flaviolin
3-linalylflaviolin + diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme activity is independent of the presence of Mg2+ or other divalent metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
addition of EDTA (10 mM) to an assay not containing any divalent cations does not affect the enzyme activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
1,3-dihydroxynaphthalene
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in 100 mM Na-TAPS (pH 8.5), at 30C
5.6
4-hydroxybenzoate
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in 100 mM Na-TAPS (pH 8.5), at 30C
0.021
flaviolin
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in 100 mM Na-TAPS (pH 8.5), at 30C
0.0021 - 0.0027
geranyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
1,3-dihydroxynaphthalene
Streptomyces cinnamonensis
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in 100 mM Na-TAPS (pH 8.5), at 30C
0.002
4-hydroxybenzoate
Streptomyces cinnamonensis
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in 100 mM Na-TAPS (pH 8.5), at 30C
0.0002
flaviolin
Streptomyces cinnamonensis
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in 100 mM Na-TAPS (pH 8.5), at 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.2
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half-maximal values at pH 7.5 and 9.2
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39100
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA-agarose resin column chromatography, benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells
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