Information on EC 2.5.1.36 - trihydroxypterocarpan dimethylallyltransferase

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The expected taxonomic range for this enzyme is: Glycine max

EC NUMBER
COMMENTARY hide
2.5.1.36
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RECOMMENDED NAME
GeneOntology No.
trihydroxypterocarpan dimethylallyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + (6aS,11aS)-3,6a,9-trihydroxypterocarpan = diphosphate + 2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan
show the reaction diagram
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dimethylallyl diphosphate + (6aS,11aS)-3,6a,9-trihydroxypterocarpan = diphosphate + 4-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkenyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glyceollin biosynthesis II
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Isoflavonoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:(6aS,11aS)-3,6a,9-trihydroxypterocarpan dimethylallyltransferase
Part of the glyceollin biosynthesis system in soy bean.
CAS REGISTRY NUMBER
COMMENTARY hide
70851-94-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the prenyltransferase is the key enzyme in the biosynthesis of prenylated flavonoids by catalyzing a rate-limiting step, i.e. the coupling process of two major metabolic pathways, the isoprenoid pathway and shikimate/polyketide pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
show the reaction diagram
dimethylallyl diphosphate + (-)-maackiain
diphosphate + ?
show the reaction diagram
poor substrate
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-
?
dimethylallyl diphosphate + genistein
diphosphate + ?
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 dimethylallyl diphosphate + 2 (6aS,11aS)-3,6a,9-trihydroxypterocarpan
2 diphosphate + (6aS,11aS)-2-dimethylallyl-3,6a,9-trihydroxypterocarpan + (6aS,11aS)-4-dimethylallyl-3,6a,9-trihydroxypterocarpan
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
50% activity
Mg2+
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100% activity; requirement for divalent cations, with Mg2+ being more effective than Mn2+
Mn2+
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68% activity; requirement for divalent cations, with Mg2+ being more effective than Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45 - 68
(6aS,11aS)-3,6a,9-Trihydroxypterocarpan
0.068
(6aS,11aS)-3,9,6a-trihydroxypterocarpan
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30°C
0.15 - 180
dimethylallyl diphosphate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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bound
Manually annotated by BRENDA team
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protein sequence is led by a plastid targeting signal sequence; sequence contains a plastid targeting signal
Manually annotated by BRENDA team
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
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sequence contains a plastid targeting signal
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed using recombinant yeast microsomes
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gene GmG4DT, sequence comparison and phylogenetic tree
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cell culture; expression of the G4DT gene is strongly up-regulated in 5 to 24 h after elicitation of phytoalexin biosynthesis in cultured soybean cells
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