Information on EC 2.5.1.50 - zeatin 9-aminocarboxyethyltransferase

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The expected taxonomic range for this enzyme is: Lupinus luteus

EC NUMBER
COMMENTARY hide
2.5.1.50
-
RECOMMENDED NAME
GeneOntology No.
zeatin 9-aminocarboxyethyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-acetyl-L-serine + zeatin = lupinate + acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminopropanoyl-group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lupinate biosynthesis
-
-
Zeatin biosynthesis
-
-
Biosynthesis of secondary metabolites
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-
SYSTEMATIC NAME
IUBMB Comments
O3-acetyl-L-serine:zeatin 2-amino-2-carboxyethyltransferase
The enzyme acts not only on zeatin but also on other N6-substituted adenines. The reaction destroys their cytokinin activity and forms the corresponding 3-(adenin-9-yl)-L-alanine.
CAS REGISTRY NUMBER
COMMENTARY hide
88068-35-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-acetyl-L-serine + 6-(2-hydroxyethylamino)purine
2-amino-3-(6-(2-hydroxyethyl)aminopurin-9yl)-propanoate + acetate
show the reaction diagram
-
20% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-(3,4-dimethoxybenzylamino)purine
2-amino-3-(6-(3,4-dimethoxybenzylamino)purin-9yl)-propanoate + acetate
show the reaction diagram
-
7.5% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-(6-hydroxyhexylamino)purine
2-amino-3-(6-aminohexylpurin-9yl)-propanoate + acetate
show the reaction diagram
-
64% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-(DELTA2-isopentenyl)aminopurine
2-amino-3-(6-(DELTA2-isopentenyl)aminopurin-9yl)-propanoate + acetate
show the reaction diagram
-
290% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-benzylaminopurine
2-amino-3-(6-benzylaminopurin-9yl)-propanoate + acetate
show the reaction diagram
-
as active as zeatin
-
-
?
O-acetyl-L-serine + 6-furfurylaminopurine
2-amino-3-(6-furfurylaminopurin-9yl)-propanoate + acetate
show the reaction diagram
-
138% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-isopentylaminopurine
2-amino-3-(6-isopentylaminopurin-9yl)-propanoate + acetate
show the reaction diagram
-
99% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-methylaminopurine
2-amino-3-(6-methylaminopurin-9-yl)propanoate + acetate
show the reaction diagram
-
25.0% of the activity with zeatin
-
-
?
O-acetyl-L-serine + 6-propylaminopurine
2-amino-3-(6-propylaminopurin-9yl)-propanoate + acetate
show the reaction diagram
-
57% of the activity with zeatin
-
-
?
O-acetyl-L-serine + adenine
2-amino-3-(6-aminopurin-9-yl)-propanoate + acetate
show the reaction diagram
-
7.5% of the activity with zeatin
-
-
?
O-acetyl-L-serine + cis-zeatin
lupinate + acetate
show the reaction diagram
-
91% of the activity with zeatin
-
-
?
O-acetyl-L-serine + dihydrozeatin
?
show the reaction diagram
-
30% of the activity with zeatin
-
-
?
O-acetyl-L-serine + O-beta-D-glucopyranosylzeatin
?
show the reaction diagram
-
34% of the activity with zeatin
-
-
?
O-acetyl-L-serine + zeatin
lupinate + acetate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-dichlorophenoxy acetic acid
-
-
2,6-dichlorophenoxy acetic acid
-
-
2-(indol-3-yl)propionic acid
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-
3-(indol-3-yl)propionic acid
-
-
4,7-dichloroindol-3-yl-acetic acid
-
-
4-chloroindol-3-yl acetic acid
-
-
5,7-dichloroindol-3-yl-acetic acid
-
-
indol-3-yl acetic acid
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-
indole-2-carboxylic acid
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-
indole-3-carboxylic acid
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-
N-(3-Chlorophenyl)-N'-phenylurea
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-
N-(3-nitrophenyl)-N'-phenylurea
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-
N-benzyl-N'-(3,4-dichlorophenyl)urea
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-
N-benzyl-N'-(3-chlorophenyl)urea
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-
N-Benzyl-N'-phenylurea
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-
phenylacetic acid
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26
adenine
-
-
0.047
O-acetyl-serine
-
-
2.5
O-glycosylzeatin
-
-
0.88
zeatin
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
2,4-dichlorophenoxy acetic acid
-
-
0.034
2,6-dichlorophenoxy acetic acid
-
-
7.2
2-(indol-3-yl)propionic acid
-
-
2.8
3-(indol-3-yl)propionic acid
-
-
0.002
4,7-dichloroindol-3-yl-acetic acid
0.0004
5,7-dichloroindol-3-yl-acetic acid
-
-
0.07
indol-3-yl acetic acid
-
-
0.14
indole-2-carboxylic acid
-
-
1.1
indole-3-carboxylic acid
-
-
0.22
N-(3-Chlorophenyl)-N'-phenylurea
-
-
0.091
N-(3-nitrophenyl)-N'-phenylurea
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-
0.023
N-benzyl-N'-(3,4-dichlorophenyl)urea
-
-
0.051
N-benzyl-N'-(3-chlorophenyl)urea
-
-
0.026
N-Benzyl-N'-phenylurea
-
-
0.065
phenylacetic acid
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64500
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gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 2-3 months, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE