Information on EC 2.5.1.71 - leachianone-G 2''-dimethylallyltransferase

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The expected taxonomic range for this enzyme is: Sophora flavescens

EC NUMBER
COMMENTARY hide
2.5.1.71
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RECOMMENDED NAME
GeneOntology No.
leachianone-G 2''-dimethylallyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dimethylallyl diphosphate + leachianone G = diphosphate + sophoraflavanone G
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sophoraflavanone G biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dimethylallyl-diphosphate:leachianone-G 2''-dimethylallyltransferase
This membrane-bound enzyme is located in the plastids and requires Mg2+ for activity. The reaction forms the lavandulyl sidechain of sophoraflavanone G by transferring a dimethylallyl group to the 2'' position of another dimethylallyl group attached at postiion 8 of leachianone G. The enzyme is specific for dimethylallyl diphosphate as the prenyl donor, as it cannot be replaced by isopentenyl diphosphate or geranyl diphosphate. Euchrenone a7 (a 5-deoxy derivative of leachianone G) and kenusanone I (a 7-methoxy derivative of leachianone G) can also act as substrates, but more slowly. Along with EC 1.14.13.103 (8-dimethylallylnaringenin 2′-hydroxylase) and EC 2.5.1.70 (naringenin 8-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone-G-biosynthesis pathway.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + euchrenone a7
diphosphate + ?
show the reaction diagram
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54% of the activity with leachianone G
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?
dimethylallyl diphosphate + leachianone G
diphosphate + sophoraflavanone G
show the reaction diagram
additional information
?
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no activity with isopentenyl diphosphate and geranyl diphosphate as prenyl donors, and with kenusanone I, 8-dimethylallylnaringenin, and isovabachin as acceptor substrates, overview
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dimethylallyl diphosphate + leachianone G
diphosphate + sophoraflavanone G
show the reaction diagram
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the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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25% of the activity with Mg2+
Co2+
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5% of the activity with Mg2+
Cu2+
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4% of the activity with Mg2+
Fe2+
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5% of the activity with Mg2+
Mg2+
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required, optimal at 10 mM, other divalent cations can only partially substitute for Mg2+, overview
Mn2+
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15% of the activity with Mg2+
Ni2+
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5% of the activity with Mg2+
Zn2+
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10% of the activity with Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.059
dimethylallyl diphosphate
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pH 8.8, 30°C
0.0023
leachianone G
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pH 8.8, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00014
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crude cell extract
0.06
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microsomes
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.9 - 10
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50% of maximal activity within this range, almost inactive at pH 6.8, pH profile, overview
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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bound, determination of enzyme distibution in membranes
Manually annotated by BRENDA team
additional information
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subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview
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Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme partially by microsome preparation
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